Journal ArticleOpen Access
Characterization of the dominant strain (G-VII) of Newcastle disease viruses isolated from commercial chickens in Bangladesh during recent outbreaks
Author Affiliations
Bangladesh Agricultural University, Bangladesh Livestock Research Institute, Gopalganj Science and Technology University
Published InJournal of Advanced Veterinary and Animal Research
Year2024
Citations4
Abstract
Objective: Newcastle disease virus genotype VII (NDV-GVII), an extremely infectious pathogen, has been causing severe economic consequences for the chicken industry. The current study aimed to isolate and characterize NDV-GVII from commercial chickens in Bangladesh during a recent outbreak. Materials and Methods: From clinically suspected chickens from 70 commercial poultry farms, a total of 420 samples (trachea, lungs, and brain tissue) were collected. The samples were cultivated in 9–10 day-old seronegative embryonated chicken eggs (ECEs) after evaluating them using the rapid Newcastle disease virus (NDV) antigen detection kit. The hemagglutination (HA) inhibition test, agar gel immune diffusion (AGID) test, molecular detection by reverse transcription-poly¬merase chain reaction (RT-PCR), and phylogenetic studies using gene sequences of fusion (F) pro¬tein. The HA pattern…
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