New insights into filamentous sludge bulking: The potential role of extracellular polymeric substances in sludge bulking in the activated sludge process

The control of filamentous sludge bulking has been regarded as an important issue in the activated sludge process due to there is still a lack of understanding of the bulking mechanisms. In this study, changes in the extracellular polymeric substances (EPS) and metabolic profile of bulking sludge based on the proteomics level was investigated to reveal the potential role of EPS in deteriorating sludge floc stability and structure during filamentous bulking. The results showed that the EPS content gradually decreased from 210.23 mg/g volatile suspended solids (VSS) to 131.34 mg/g VSS during sludge bulking. The protein (PN) content of the EPS significantly decreased from 173.33 mg/g VSS to 95.42 mg/g VSS during sludge bulking. However, a gradual increase in polysaccharides…