Functional analysis of extracellular polymeric substances (EPS) during the granulation of aerobic sludge: Relationship among EPS, granulation and nutrients removal

Extracellular polymeric substances (EPS) with high molecular weights, secreted from microorganisms, play a critical functional role in the aerobic granular sludge (AGS). To investigate the level and function of EPS during the granulation of aerobic sludge and in the mature AGS, a sequencing batch reactor (SBR) was operated for 70 days. Aerobic granules with average diameters of 0.25 mm were obtained with reduced settling time of sludge. Simultaneous removals of COD, nitrogen and phosphorus by the mature AGS exceeded 90, 95 and 90%, respectively. The EPS content increased significantly to above 333 mg/g MLVSS during the initial stage, and after that, it stabilized at about 240 mg/g MLVSS as the mature AGS formed, higher than that of the seed sludge…