Preliminary Studies on Palladium Nanoparticle as a Novel Label for DNA Microarray and Their Corresponding Detection

This paper firstly describes the preliminary results achieved by using palladium nanoparticle (Pd NP) as a novel label for the detection of DNA hybridization in DNA microarray. And two signal amplification procedures based on "the silver staining" or "the cobalt staining" are presented during above analysis. The results show that the label Pd NP-ssDNA (target) (single strand DNA(target)) performs high single base pair mismatch-discrimination capability. The succeeding silver staining or cobalt staining procedure greatly amplifies such a signal through the catalysis of Pd. For "the silver staining:' the background staining is very low and the silver deposition only occurs around Pd NPs. So such a procedure provides a alternative for "Gold Label Silver Stain" presented by Mirkin C. A. For…