Partial Purification of Deamidase from Germinating Wheat (<i>Triticum aestivum</i> L.) Seeds and its Application for the Improvement of Functional Properties of Wheat Gluten

Enzyme with deamidase activity was found in the germinating wheat seeds. The activity appeared in the seeds after 24 hours of germination and reached its maximum value after 48 hours and then declined rapidly. Protein deamidase from germinating wheat seeds was further purified by salting out with phosphate salts and by subsequent chromatography on gel filtration and DEAE-cellulose column chromatography. Polyacrylamide slab gel elec trophoresis showed the purified enzyme to be homogeneous. The molecular weight was determined to be 59 kDa by gel filtration and 60 kDa, by SDS-Polyacrylamide slab gel electrophoresis (SDS-PAGE). The optimum pH and temperature of the purified enzyme were found to be 6.9 and 32°C, respectively.The functional properties of wheat gluten were investigated after treating with…