Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots.

OBJECTIVE: The present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of Azadirachta indica (A. indica). MATERIALS AND METHODS: To evaluate the antioxidant properties of the crude extract, some complementary test systems, namely DPPH free radical scavenging assay, reducing power assay, and ferrous ion chelating ability and determination of total phenolic content were conducted. RESULTS: In DPPH free radical scavenging test, IC50 value of the crude extract was found to be fairly significant (13.81±0.06 μg/ml) while compared with that of the reference standards, ascorbic acid and BHA (2.12±0.02 and 4.87±0.05 μg/ml, respectively). In reducing power assay, the maximum absorbance for the extract was found to be 1.523±0.026 at100…