T2182C Mutation Is Not Associated with Clarithromycin Resistance in <i>Helicobacter pylori</i>

T2182C Mutation Is Not Associated with Clarithromycin Resistance in Helicobacter pyloriIn a recent article, Khan et al. described a new mutation in the 23S rRNA gene, T2182C, conferring clarithromycin resistance to Helicobacter pylori (1).An A-to-G mutation at either position 2142 or position 2143 and an A-to-C mutation at position 2142 in the 23S rRNA gene have been shown to confer resistance to clarithromycin in H. pylori (5).In 2000, we isolated a clarithromycin-resistant (Cla r ) strain from a French patient (MIC Ͼ 256 g/ml).Sequencing the 23S rRNA gene revealed a single T2182C mutation.To confirm the role of the T2182C mutation, we used the PCR fragment containing this mutation to transform a susceptible receptor strain.We obtained Cla r transformants.These results were…