Integrated Immuno and bioinformatics assisted novel epitope vaccine against HIV infection: a study based on complete genome

As the HIV-based complication is still going on with the high infection and mortality rate, it requires a novel strategy to combat this infection due to the unavailability of proper therapeutic. Therefore, we utilize integrated immuno and bioinformatics approaches in this study to design a peptide vaccine against HIV infection by targeting its complete genome. The complete genome sequence was analyzed, and the potential B and T cells were predicted. Among the predicted epitopes, the promising ones were selected and further used with the adjuvant and linker to formulate a vaccine candidate. The vaccine was modeled, and its activity and stability towards the TLRs were analyzed via docking and dynamics (500ns). The vaccine-generated immune activity and expression via in-silico cloning…