Journal ArticleOpen Access
Proteomic Analysis of the Cyst Stage of Entamoeba histolytica
Authors
Author Affiliations
University of Virginia Health System, International Centre for Diarrhoeal Disease Research, University of Virginia, Center for Infectious Disease Research
Published InPLoS neglected tropical diseases
Year2012
Citations42
Abstract
BACKGROUND: The category B agent of bioterrorism, Entamoeba histolytica has a two-stage life cycle: an infective cyst stage, and an invasive trophozoite stage. Due to our inability to effectively induce encystation in vitro, our knowledge about the cyst form remains limited. This also hampers our ability to develop cyst-specific diagnostic tools. AIMS: Three main aims were (i) to identify E. histolytica proteins in cyst samples, (ii) to enrich our knowledge about the cyst stage, and (iii) to identify candidate proteins to develop cyst-specific diagnostic tools. METHODS: Cysts were purified from the stool of infected individuals using Percoll (gradient) purification. A highly sensitive LC-MS/MS mass spectrometer (Orbitrap) was used to identify cyst proteins. RESULTS: A total of 417 non-redundant E. histolytica…
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