Phytochemical screening and evaluation of pharmacological activity of leaf Methanolic extract of Colocasia affinis Schott

The purpose of the current study is to identify the phytochemicals as well as to determine the level of antioxidant, cytotoxic, antimicrobial and in vivo analgesic, anti-inflammatory and antidiarrheal activity of leaf methanolic extract of Colocasia affinis Schott (LMCA). To appraise the antioxidant activities the extensively used total phenol, flavonoid, total antioxidant capacity, ferric reducing antioxidant power, cupric reducing antioxidant capacity and DPPH scavenging assay have been used. Cytotoxic potential was determined by brine shrimp lethality test whereas antimicrobial activity was assessed by disc diffusion method. Acetic acid induced writhing and formalin induced paw licking methods were employed to evaluate analgesic activity. To gauge anti-inflammatory activity, xylene-induced ear edema and carrageenan induced paw edema methods were used. Castor oil induced…