Callus Induction and Virus Free Potato Mini-Tuber Production through Meristem Culture

The experiments were conducted with a view to establish a protocol for virus free mini-tubers production, in vitro, using potato meristem as explant, collected from potato sprouts of three popular potato varieties Diamant, Cardinal, Granola. In the present study four levels of GA3 (100, 200, 300 and 400 ppm) were used to assess the influence on sprouting abilities in three selected potato verities. The maximum sprouting efficiency was observed in 400ppm GA3 treatment within short period of time in Granola variety. The effect of different combination and concentration of IBA, 2, 4-D and BAP was used along with fresh MS media to inoculate meristems of potato sprouts. IBA concentration were 0.25mg/L, 1.5mg/L; 2, 4-D concentration were 0.125mg/L, 0.25mg/L, 0.37mg/L, 0.50mg/L,…