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Multiplex amplicon sequencing dataset for genotyping pandemic populations of the wheat blast fungus

Author Affiliations
Golden Valley Agricultural Research Trust, Kobe University, Sainsbury Laboratory, Centro Internacional de Mejoramiento de Maíz Y Trigo, ...
Published InZenodo (CERN European Organization for Nuclear Research)
Year2021

Abstract

<strong>We report genotyping data for 186 <em>Magnaporthe oryzae</em> (Syn. <em>Pyricularia oryzae</em>) isolates from different geographical regions obtained using multiplex amplicon sequencing. The dataset consists of 84 single nucleotide polymorphisms (SNPs) selected from transcript sequences to distinguish between the clonal lineage of the wheat blast fungus that emerged in South East Asia in 2016 and other <em>M. oryzae</em>genotypes. The sample coverage includes pandemic wheat blast isolates from Bangladesh (N=75, 2016-2020) and the newly reported Zambian outbreak (N=13, 2018-2020). The dataset is provided as an open science resource as part of the OpenWheatBlast initiative. We welcome analyses and feedback from the community.</strong>
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