Journal ArticleOpen Access
Multiplex PCR Assay for Rapid Detection and Genotyping of <i>Helicobacter pylori</i> Directly from Biopsy Specimens
Author Affiliations
National Institute of Cholera and Enteric Diseases, Institute of Post Graduate Medical Education and Research, Washington University in St. Louis, International Centre for Diarrhoeal Disease Research
Published InJournal of Clinical Microbiology
Year2004
Citations123
Abstract
We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.
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