Journal ArticleOpen Access
A genome-wide DNA methylation study in colorectal carcinoma
Authors
Author Affiliations
Chicago Department of Public Health, University of Chicago, Bangabandhu Sheikh Mujib Medical University, University of Chicago Research Bangladesh
Published InBMC Medical Genomics
Year2011
Citations69
Abstract
BACKGROUND: We performed a genome-wide scan of 27,578 CpG loci covering 14,475 genes to identify differentially methylated loci (DML) in colorectal carcinoma (CRC). METHODS: We used Illumina's Infinium methylation assay in paired DNA samples extracted from 24 fresh frozen CRC tissues and their corresponding normal colon tissues from 24 consecutive diagnosed patients at a tertiary medical center. RESULTS: We found a total of 627 DML in CRC covering 513 genes, of which 535 are novel DML covering 465 genes. We also validated the Illumina Infinium methylation data for top-ranking genes by non-bisulfite conversion q-PCR-based methyl profiler assay in a subset of the same samples. We also carried out integration of genome-wide copy number and expression microarray along with methylation profiling…
View at Publisher
BORR does not host full-text PDFs. The button above takes you to the original publisher.