Assembly and interactions of denitrifying and anammox communities in a typical eutrophic lake

Nitrogen removal is crucial for controlling nitrogen levels in eutrophic lakes and depends on the high transformation capacity of nitrogen-cycling microorganisms. However, the assembly mechanisms and interactions of nitrogen removal microbial communities in lake water remain unclear. This study aimed to clarify how nitrogen levels influence the diversity, interactions, and assembly of denitrifying and anaerobic ammonia oxidation (anammox) communities. We collected lake water from different areas in a typical eutrophic lake and investigated the nitrogen removal bacterial communities by high-throughput sequencing of two representative functional genes (nirS and hzsB). Our results indicated that the α-diversity of anammox bacteria was higher in sites with high concentrations of nitrogen (>1.5 mg/l) than in sites with low concentrations of nitrogen (<1.5 mg/l). Anammox…