OtherOpen Access
Comparative Phylogenetic Analysis and Transcriptomic Profiling of Dengue (DENV-3 genotype I) Outbreak in 2021 in Bangladesh
Author Affiliations
Bangladesh Council of Scientific and Industrial Research, University of Dhaka, Noakhali Science and Technology University, Jagannath University
Published InbioRxiv (Cold Spring Harbor Laboratory)
Year2022
Citations1
Abstract
Abstract Objectives The lack of a dengue disease animal model and the complex immune interaction in dengue infection hinders the study of host response and immunopathogenesis. The development of next-generation sequencing (NGS) technology allowed the researchers to study the transcriptomic profiles of human in-depth. We, therefore, implicated phylodynamic and transcriptomic approaches through NGS technology to know the origin of dengue virus (DENV) and their host response in infected patients with dengue fever. Methods To determine the whole genome sequences of the dengue virus and their transcriptomic profiles, RNA was extracted from the serum samples of 3 healthy, and 21 dengue patients. These samples were custom performed at phylogenetic, phylodynamic, differential express gene (DEG), and gene ontology (GO) using respective bioinformatics…
View at Publisher
BORR does not host full-text PDFs. The button above takes you to the original publisher.