Nitrogen absorption efficiency and mechanism in Arbuscular mycorrhizal fungi - Canna indica symbiosis

Arbuscular mycorrhizal fungi (AMF) and its symbiosis with Canna indica on nitrogen (N) absorption was investigated for the remediation of contaminated soil. Canna indica plants with rhizome and leaf integrity intact were collected in spring and autumn seasons. To maintain the ideal nutrient composition, Hoagland concentrated nutrient solution was diluted with deionized water and additional nutrient solution was added periodically. Treated root samples were observed with an optical microscope and the number of hyphae and intersections as well as inoculation status were examined. High-throughput sequencing experiment was conducted to quantify AMF inoculation. Alpha diversity study was used to characterize abundance and diversity of the symbiosis. Hydroponic experiments were conducted to explore the absorption effectiveness of AMF-Canna symbiosis under different NH…