Mature biofilm-sensitive lysozyme-grafted Bi-guanidine backbone porphyrin nanorods for deep penetration and double phototherapy

The efficacy of antibacterial therapy was largely vitiated because of the shield of bacteria by the intricate architecture of biofilms. For emerging phototherapy strategies like photothermal therapy (PTT) and photodynamic therapy (PDT), dense biofilms can substantially impede the permeation of photo-agents, ultimately compromising the thermal conductivity and reactive oxygen species (ROS) diffusion. To address these challenges, we have proposed a biofilm-sensitive MT nanorod with dual phototherapy, based on the ordered directional assembly of photosensitizer meso-tetra(4-carboxyphenyl) porphine (TCPP) molecules by metformin hydrochloride (MET) as functional backbone. The formed MT nanorod avoids the uncontrollable aggregation of TCPP, producing pleased water-solubility with efficient fluorescent emission and allowing simultaneous PTT-PDT effects under a single laser. The grafted MT-LYZ by conjugation of lysozyme (LYZ) to…