S L Moseley, I. Huq, A. R. M. A. Alim, Magdalene So et al.
A method fo detecting large numbers of isolates of enterotoxigenic Escherichia coli is described in which the genes encoding th enterotoxins are detected, rather than the toxins themselves. Radiolabeled fragments of DNA encoding the heat-labile (LT) or heat-stable (ST) toxins were used as hybridizat...
Amita Sharma, Ayub Qadri
Vi capsular polysaccharide (Vi) was first identified as a virulence antigen of Salmonella typhi, the causative agent of typhoid fever in humans; it renders S. typhi resistant to phagocytosis and the action of serum complement. However, the role of Vi during the infection of intestinal epithelium wit...
Jianquan Lu, Jie Zhong, Daniel W. C. Ho, Yang Tang et al.
This paper is devoted to studying the trajectory and state controllability of Boolean control networks (BCNs) with time delay. In contrast to BCNs without time delay, the dynamics of delayed BCNs are determined by a sequence of initial states, named here trajectories. Trajectory controllability mean...
Yogesh Hooda, Mohammad Saiful Islam Sajib, Hafizur Rahman, Stephen P. Luby et al.
BACKGROUND: With the rise in fluoroquinolone-resistant Salmonella Typhi and the recent emergence of ceftriaxone resistance, azithromycin is one of the last oral drugs available against typhoid for which resistance is uncommon. Its increasing use, specifically in light of the ongoing outbreak of exte...
Ghadah Alsharif, Sadia Ahmad, Md. Shahidul Islam, Riddhi Shah et al.
Enterohemorrhagic Escherichia coli (EHEC) is a foodborne pathogen causing hemorrhagic colitis and hemolytic uremic syndrome. EHEC colonizes the intestinal tract through a range of virulence factors encoded by the locus of enterocyte effacement (LEE), as well as Shiga toxin. Although the factors invo...
Masatomo Morita, Makoto Ohnishi, Eiji Arakawa, N. A. Bhuiyan et al.
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
Firdausi Qadri, Sk Arafat Hossain, I Ciznár, K Haider et al.
Smooth strains of Shigella dysenteriae type 1, Shigella flexneri, Shigella boydii, and Shigella sonnei which form pigmented colonies (Pcr+) on Congo red agar were virulent in the Sereny test. Smooth variants unable to bind Congo red (Pcr-) were avirulent. Measurements of dye uptake from solution sho...
Xiangmin Lin, Li-qun Kang, Hui Li, Xuan‐xian Peng
Bacterial antibiotic resistance has become a worldwide challenge with the overuse and misuse of drugs. Several mechanisms for the resistance are revealed, but information regarding the bacterial global response to antibiotics is largely absent. In this study, we characterized the differential proteo...
Megan E. Carey, Ruby Jain, M. Yousuf, Mailis Maes et al.
BACKGROUND: The emergence and spread of antimicrobial resistance (AMR) pose a major threat to the effective treatment and control of typhoid fever. The ongoing outbreak of extensively drug-resistant Salmonella Typhi (S. Typhi) in Pakistan has left azithromycin as the only remaining broadly efficacio...
S. Navaratnam, G M Myles, Richard W. Strange, Aziz Sancar
The UvrA protein is the damage recognition subunit of the Escherichia coli repair enzyme ABC excision nuclease. Sequence analysis of this 940-amino acid protein revealed two regions of sequence homology to the zinc finger motif found in many DNA binding proteins. Physical and chemical analyses indic...
Koushik Roy, Scott R. Bartels, Firdausi Qadri, James M. Fleckenstein
Enterotoxigenic Escherichia coli (ETEC) causes considerable morbidity and mortality due to diarrheal illness in developing countries, particularly in young children. Despite the global importance of these heterogeneous pathogens, a broadly protective vaccine is not yet available. While much is known...
Faisal Bin Rahman, Bishajit Sarkar, Ripa Moni, Mohammad Shahedur Rahman
Surfactin is a biosurfactant produced by Bacillus subtilis. The srfA operon, Sfp gene, and two quorum sensing systems are required for its production. The master regulator spo0A also plays an indispensable role in proper surfactin synthesis. Upon production, surfactin itself acts as a signaling mole...
Masahiro Asakura, Atsushi Hinenoya, Mohammad Samiul Alam, Kensuke Shima et al.
Cytolethal distending toxins (CDTs) are inhibitory cyclomodulins, which block eukaryotic cell proliferation and are produced by a diverse group of Gram-negative bacteria, including Escherichia coli strains associated with intestinal and extraintestinal infections. However, the mode of transmission o...
Richelle C. Charles, Jason B. Harris, Michael R. Chase, Lauren M. Lebrun et al.
BACKGROUND: S. Typhi, a human-restricted Salmonella enterica serovar, causes a systemic intracellular infection in humans (typhoid fever). In comparison, S. Typhimurium causes gastroenteritis in humans, but causes a systemic typhoidal illness in mice. The PhoP regulon is a well studied two component...
Atsushi Hinenoya, Kensuke Shima, Masahiro Asakura, Kazuhiko Nishimura et al.
BACKGROUND: Cytolethal distending toxin (CDT)-producing Escherichia coli (CTEC) has been isolated from patients with gastrointestinal or urinary tract infection, and sepsis. However, the source of human infection remains unknown. In this study, we attempted to detect and isolate CTEC strains from fe...