L. D. Wright, H. R. Skegs
The microbiological determination of biotin, using Lactobacillus arabinosus as the test organism, was studied. The existence of a water-soluble, avidin-combinable, form of biotin in certain enzymatically prepared materials which is available to L. casei, but not to L. arabinosus, was demonstrated. W...
M. Steven Oberste, Kaija Maher, A. J. Williams, Naomi Dybdahl-Sissoko et al.
The 65 serotypes of human enteroviruses are classified into four species, Human enterovirus (HEV) A to D, based largely on phylogenetic relationships in multiple genome regions. The 3'-non-translated region of enteroviruses is highly conserved within a species but highly divergent between species. F...
Sorin Istrail, Granger G. Sutton, Liliana Florea, Aaron L. Halpern et al.
We report a whole-genome shotgun assembly (called WGSA) of the human genome generated at Celera in 2001. The Celera-generated shotgun data set consisted of 27 million sequencing reads organized in pairs by virtue of end-sequencing 2-kbp, 10-kbp, and 50-kbp inserts from shotgun clone libraries. The q...
Maurice W. Southworth, Eric Adam, Daniel Panne, Robyn Byer et al.
Inteins are protein splicing elements that mediate their excision from precursor proteins and the joining of the flanking protein sequences (exteins). In this study, protein splicing was controlled by splitting precursor proteins within the Psp Pol-1 intein and expressing the resultant fragments in ...
Mohammad Saifur Rahman, Swakkhar Shatabda, Sanjay Saha, M. Kaykobad et al.
A DNA-binding protein (DNA-BP) is a protein that can bind and interact with a DNA. Identification of DNA-BPs using experimental methods is expensive as well as time consuming. As such, fast and accurate computational methods are sought for predicting whether a protein can bind with a DNA or not. In ...
Sumaiya Iqbal, Eduardo Pérez‐Palma, Jakob Berg Jespersen, Patrick May et al.
Interpretation of the colossal number of genetic variants identified from sequencing applications is one of the major bottlenecks in clinical genetics, with the inference of the effect of amino acid-substituting missense variations on protein structure and function being especially challenging. Here...
Md Mehedi Hasan, Shiping Yang, Yuan Zhou, Md. Nurul Haque Mollah
Lysine succinylation is an emerging protein post-translational modification, which plays an important role in regulating the cellular processes in both eukaryotic and prokaryotic cells. However, the succinylation modification site is particularly difficult to detect because the experimental technolo...
Chunyou Mao, Peng Xiao, Xiao-Na Tao, Jiao Qin et al.
Individual free fatty acids (FAs) play important roles in metabolic homeostasis, many through engagement with more than 40G protein–coupled receptors. Searching for receptors to sense beneficial omega-3 FAs of fish oil enabled the identification of GPR120, which is involved in a spectrum of metaboli...
Shahana Yasmin Chowdhury, Swakkhar Shatabda, Abdollah Dehzangi
DNA-binding proteins play a very important role in the structural composition of the DNA. In addition, they regulate and effect various cellular processes like transcription, DNA replication, DNA recombination, repair and modification. The experimental methods used to identify DNA-binding proteins a...
Ming Li, Congjiao Sun, Naiyi Xu, Peipei Bian et al.
Abstract The gene numbers and evolutionary rates of birds were assumed to be much lower than those of mammals, which is in sharp contrast to the huge species number and morphological diversity of birds. It is, therefore, necessary to construct a complete avian genome and analyze its evolution. We co...
Betty A. Brown, Kaija Maher, Mary R. Flemister, Pejman Naraghi‐Arani et al.
Molecular methods, based on sequencing the region encoding the VP1 major capsid protein, have recently become the gold standard for enterovirus typing. In the most commonly used scheme, sequences more than 75% identical (>85% amino acid identity) in complete or partial VP1 sequence are considered to...
Vincent V. Pham, Carolina Salguero, Shamsun Nahar Khan, Jennifer L. Meagher et al.
The HIV Tat protein competes with the 7SK:HEXIM interaction to hijack pTEFb from 7SK snRNP and recruit it to the TAR motif on stalled viral transcripts. Here we solve structures of 7SK stemloop-1 and TAR in complex with Tat's RNA binding domain (RBD) to gain insights into this process. We find that ...
Md. Khaledur Rahman, M. Sohel Rahman
The CRISPR/Cas9-sgRNA system has recently become a popular tool for genome editing and a very hot topic in the field of medical research. In this system, Cas9 protein is directed to a desired location for gene engineering and cleaves target DNA sequence which is complementary to a 20-nucleotide guid...
Rumana Mahtarin, Shafiqul Islam, Md. Jahirul Islam, M. Obayed Ullah et al.
) approach with Robetta and trRosetta servers. Comparing with other model structures, it is evident that trRosetta (TM-score: 0.64; TM region RMSD: 2 Å) can provide the best model than Robetta (TM-score: 0.61; TM region RMSD: 3.3 Å) and I-TASSER (TM-score: 0.45; TM region RMSD: 6.5 Å). 100 ns molecu...
Xiangmin Lin, Li-qun Kang, Hui Li, Xuan‐xian Peng
Bacterial antibiotic resistance has become a worldwide challenge with the overuse and misuse of drugs. Several mechanisms for the resistance are revealed, but information regarding the bacterial global response to antibiotics is largely absent. In this study, we characterized the differential proteo...