Jelle Matthijnssens, Max Ciarlet, Sarah M. McDonald, Houssam Attoui et al.
In April 2008, a nucleotide-sequence-based, complete genome classification system was developed for group A rotaviruses (RVs). This system assigns a specific genotype to each of the 11 genome segments of a particular RV strain according to established nucleotide percent cutoff values. Using this app...
Jelle Matthijnssens, Max Ciarlet, Erica Heiman, Ingrid Arijs et al.
Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system...
Jie Liu, Jean Gratz, Caroline Amour, Gibson Kibiki et al.
The TaqMan Array Card (TAC) system is a 384-well singleplex real-time PCR format that has been used to detect multiple infection targets. Here we developed an enteric TaqMan Array Card to detect 19 enteropathogens, including viruses (adenovirus, astrovirus, norovirus GII, rotavirus, and sapovirus), ...
Bárbara Brito, Luis L. Rodrı́guez, J. M. Hammond, Julio Pinto et al.
Foot-and-mouth disease (FMD) virus affects livestock worldwide. There are seven different serotypes, each with a diversity of topotypes, genetic lineages and strains. Some lineages have different properties that may contribute to sporadic spread beyond their recognized endemic areas. The objective o...
Lili Zhang, Daniel A. Zlotoff, Magid Awadalla, Syed Mahmood et al.
Introduction: myocarditis is a potentially fatal complication of immune checkpoint inhibitors (ICI). While corticosteroids are the cornerstones of the treatment, there are no data to guide the dose and timing.\n\nMethods: from an international registry of patients with ICI myocarditis diagnosed betw...
Caitlin Naylor, Miao Lu, Rashidul Haque, Dinesh Mondal et al.
BACKGROUND: Environmental enteropathy (EE) is a subclinical enteric condition found in low-income countries that is characterized by intestinal inflammation, reduced intestinal absorption, and gut barrier dysfunction. We aimed to assess if EE impairs the success of oral polio and rotavirus vaccines ...
Jelle Matthijnssens, Mustafizur Rahman, Vito Martella, Yang Xuelei et al.
The Belgian rotavirus strain B4106, isolated from a child with gastroenteritis, was previously found to have VP7 (G3), VP4 (P[14]), and NSP4 (A genotype) genes closely related to those of lapine rotaviruses, suggesting a possible lapine origin or natural reassortment of strain B4106. To investigate ...
Mark Zeller, Mustafizur Rahman, Elisabeth Heylen, Sarah De Coster et al.
Rotarix™ was introduced into the Belgian market in 2006 and RotaTeq™ in 2007, quickly reaching more than 85% vaccine coverage of all newborns in Belgium. The incidence of rotavirus gastroenteritis has been monitored in the Gasthuisberg University Hospital (GUH), Belgium since 1986, and since 1999 th...
Jelle Matthijnssens, Christiaan A. Potgieter, Max Ciarlet, Viviana Parreño et al.
A limited number of human G6P[14] rotavirus strains that cause gastroenteritis in humans have been isolated in Europe and Australia. The complete genome sequences were determined for five of these human strains--B10925-97 (isolated in Belgium in 1997), 111/05-27 (Italy, 2005), PA169 (Italy, 1987), M...
Leanne Unicomb, Goutam Podder, Jon R. Gentsch, Patricia Woods et al.
We characterized 1,534 rotavirus (RV) strains collected in Bangladesh from 1992 to 1997 to assess temporal changes in G type and to study the most common G and P types using reverse transcription-PCR, oligonucleotide probe hybridization, and monoclonal antibody-based enzyme immunoassay. Results from...
M. Steven Oberste, Kaija Maher, A. J. Williams, Naomi Dybdahl-Sissoko et al.
The 65 serotypes of human enteroviruses are classified into four species, Human enterovirus (HEV) A to D, based largely on phylogenetic relationships in multiple genome regions. The 3'-non-translated region of enteroviruses is highly conserved within a species but highly divergent between species. F...
Mustafizur Rahman, Rasheda Sultana, Giasuddin Ahmed, Sharifun Nahar et al.
Approximately 20,000 stool specimens from patients with diarrhea visiting 1 urban and 1 rural hospital in Bangladesh during January 2001-May 2006 were tested for group A rotavirus antigen, and 4,712 (24.0%) were positive. G and P genotyping was performed on a subset of 10% of the positive samples (n...
Joseph Bresee, Zhao‐yin Fang, Bei Wang, E. Anthony S. Nelson et al.
Rotavirus remains the most common cause of severe, dehydrating diarrhea among children worldwide. Several rotavirus vaccines are under development. Decisions about new vaccine introduction will require reliable data on disease impact. The Asian Rotavirus Surveillance Network, begun in 2000 to facili...
M. Steven Oberste, Kaija Maher, Suzanne M. Michele, Gaël Belliot et al.
Molecular methods have enabled the rapid identification of new enterovirus (EV) serotypes that would have been untypable using existing neutralizing antisera. Nineteen strains of four new EV types termed EV76 (11 isolates), EV89 (two isolates), EV90 (four isolates) and EV91 (two isolates), isolated ...
Mustafizur Rahman, Jelle Matthijnssens, Sharifun Nahar, Goutam Podder et al.
A novel rotavirus strain (Dhaka6) isolated from a 21-year-old Bangladeshi male patient was characterized by sequence analysis of its VP7 and VP4 gene segments. Phylogenetic analysis of the VP7 gene of the Dhaka6 strain revealed a common evolutionary lineage with porcine G11 rotavirus strains. This i...