John D. Clemens, David A. Sack, Jeffrey R. Harris, J. Chakraborty et al.
The B subunit (BS) of cholera toxin and that of the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC) are antigenically similar. We therefore assessed whether a combined cholera toxin BS/whole-cell (BS-WC) oral vaccine against cholera conferred cross-protection against LT-produ...
A.‐M. Svennerholm, Marianne Jertborn, I. e. Gothefors, Amr M. Karim et al.
Mucosal and systemic immune responses to a new oral cholera vaccine, consisting of the B subunit plus killed vibrios, were studied in Bangladeshi volunteers and compared with those to clinical cholera. A single peroral dose of vaccine induced a local IgA antitoxin response in intestinal-lavage fluid...
Firdausi Qadri, Swadesh K. Das, Abu Syed Golam Faruque, George J. Fuchs et al.
The prevalence of toxin types and colonization factors (CFs) of enterotoxigenic Escherichia coli (ETEC) was prospectively studied with fresh samples (n = 4,662) obtained from a 2% routine surveillance of diarrheal stool samples over 2 years, from September 1996 to August 1998. Stool samples were tes...
Sandra Panchalingam, Martín Antonio, Anowar Hossain, Inácio Mandomando et al.
To understand the etiology of moderate-to-severe diarrhea among children in high mortality areas of sub-Saharan Africa and South Asia, we performed a comprehensive case/control study of children aged <5 years at 7 sites. Each site employed an identical case/control study design and each utilized a u...
Robert E. Black, Myron M. Levine, M L Clements, Charles R. Young et al.
Natural protection from cholera is associated with local intestinal antibacterial and antitoxic antibodies, which appear to act synergistically. Although current parenteral cholera vaccines offer insufficient protection, new vaccines administered orally have more promise. Killed Vibrio cholerae, alo...
J. D. Clemens, Jeffrey R. Harris, David A. Sack, J. Chakraborty et al.
We assessed the protective efficacy (PE) of three doses of B subunit-killed whole cell (BS-WC) and killed whole cell-only (WC) oral cholera vaccines in a randomized, double-blind trial among 62,285 children and women residing in rural Bangladesh. After one complete year of surveillance, 110 cases of...
Firdausi Qadri, Christine Wennerås, M. John Albert, Jaber Hossain et al.
Vibrio cholerae O139 has recently emerged as the second etiologic agent of cholera in Asia. A study was carried out to evaluate the induction of specific immune responses to the organism in V. cholerae O139-infected patients. The immune responses to V. cholerae O139 Bengal were studied in patients b...
F.P.L. van Loon, John D. Clemens, J. Chakraborty, Malla Rao et al.
To determine the protective efficacy (PE) of three doses of oral B subunit-killed whole cell (BS-WC) or killed whole cell-only (WC) vaccines against cholera, a clinical trial was conducted among 62285 children over 2 years and adult women in rural Bangladesh. During 5 years of follow-up, there were ...
F. Qadri, T R Bhuiyan, K K Dutta, R Raqib et al.
BACKGROUND AND AIMS: The general concept is that as Vibrio cholerae is not invasive, it mediates a non-inflammatory type of infection. This is being re-evaluated based on available data that natural cholera infection or cholera toxin induces a Th2-type of immune profile and stimulates the humoral im...
John D. Clemens, Bonita Stanton, J. Chakraborty, David A. Sack et al.
We conducted a randomized trial among persons in rural Bangladesh to evaluate the side effects and immunogenicity of orally administered B subunit-killed whole cell (BS-WC) and killed whole cell-only (WC) cholera vaccines and a killed Escherichia coli strain K12 placebo proposed for field testing. T...
Firdausi Qadri, Golam Mohi, Jaber Hossain, Tasnim Azim et al.
Vibrio cholerae serogroup O139, now considered to be the second organism capable of causing epidemic severe dehydrating cholera, contains a capsular polysaccharide which makes it difficult for it to be used in the conventional vibriocidal antibody assay optimized for V. cholerae O1. After modificati...
A.‐M. Svennerholm, Jan Holmgren, R.A. Black, Myron M. Levine et al.
A ganglioside enzyme-linked immunosorbent assay (ELISA) was used to study and attempt to differentiate between antitoxin responses in persons infected with either Vibrio cholerae or Escherichia coli producing heat-labile enterotoxin. In most cases (69%-94%), experimentally infected North Americans a...
Leif Gothefors, C Ahrén, Barbara J. Stoll, D Barua et al.
In Dhaka, Bangladesh, fresh isolates of Escherichia coli from 197 patients with diarrhea were investigated for production of enterotoxin and possession of colonization factor antigen (CFA) I or II. Enterotoxigenic E. coli (ETEC) was isolated from 34% of the patients, and of the 67 enterotoxin-positi...
A.‐M. Svennerholm, Jan Holmgren, David A. Sack, P K Bardhan
In about 80% of Bangladeshi volunteers a single oral or intramuscular immunisation with a new cholera toxoid immunogen (B subunit) gave rise to a local intestinal secretory immunoglobulin A (IgA) antitoxin response as measured in intestinal-lavage fluid by enzyme-linked immunosorbent assay methods. ...
John D. Clemens, J.D. Clemens, F. van Loon, D.A. Sack et al.
To test the hypothesis that clinical Vibrio cholerae O1 infections protect against recurrent cholera, treated cholera episodes in a rural Bangladesh population of 188,153 people who were followed between 1985 and 1988 were analysed. Of the 2214 people with initial episodes of cholera, 7 had a second...
Yasmin Ara Begum, Kaisar A. Talukder, Ishrat J. Azmi, Mohammad Shahnaij et al.
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children as well as in travellers to ETEC endemic countries. Ciprofloxacin is a broad-spectrum antimicrobial agent nowadays used for the treatment of dia...
Barbara J. Stoll, A.‐M. Svennerholm, Leif Gothefors, D Barua et al.
Fifteen patients hospitalized with acute, watery diarrhea and with enterotoxigenic Escherichia coli (ETEC) detected from stool samples were studied to evaluate the extent to which natural ETEC diarrhea induces local and systemic antibody responses to E. coli heat-labile toxin (LT), homologous lipopo...
David A. Sack, John D. Clemens, S. Huda, Jeffrey R. Harris et al.
Sera collected during the 1985 oral cholera vaccine trial in Matlab, Bangladesh, which demonstrated efficacy of a whole cell combined with cholera B subunit vaccine (WC/BS) and a whole cell only vaccine (WC), were analyzed for antitoxin and vibriocidal antibodies. Before vaccines were given, antitox...
Tanvir Ahmed, Anna Lundgren, Mohammad Arifuzzaman, Firdausi Qadri et al.
Recent studies have shown that children with blood group A have increased susceptibility to enterotoxigenic Escherichia coli (ETEC) diarrhea and that Lewis blood group "a" antigen (Le(a)) may be a candidate receptor for ETEC colonization factor (CF) antigen I (CFA/I) fimbriae. Based on these finding...
Sujit Bhattacharya, Robert E. Black, Lawrence L. Bourgeois, John D. Clemens et al.
Long-lasting cholera outbreaks in Africa suggest limitations in the current strategy of disease control.