Amy H.Y. Tong, Marie Evangelista, Ainslie B. Parsons, Hong Xu et al.
In Saccharomyces cerevisiae, more than 80% of the approximately 6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synt...