Michelle A. Peck, Kimberly Sturk‐Andreaggi, Jacqueline Tyler Thomas, Robert S. Oliver et al.
Generating mitochondrial genome (mitogenome) data from reference samples in a rapid and efficient manner is critical to harnessing the greater power of discrimination of the entire mitochondrial DNA (mtDNA) marker. The method of long-range target enrichment, Nextera XT library preparation, and Illum...
Erin M. Gorden, Kimberly Sturk‐Andreaggi, Charla Marshall
DNA sequence damage from cytosine deamination is well documented in degraded samples, such as those from ancient and forensic contexts. This study examined the effect of a DNA repair treatment on mitochondrial DNA (mtDNA) from aged and degraded skeletal samples. DNA extracts from 21 non-probative, d...
J. Ring, Kimberly Sturk‐Andreaggi, Michelle A. Peck, Charla Marshall
Nuclear mitochondrial DNA segments (NUMTs) have arisen because of the transposition of segments of the mitochondrial DNA genome (mitogenome) into the nuclear genome. When using a "mitotiling" strategy, NUMTs may be more readily amplified when targeting the entire mitogenome compared to the control r...
Erin M. Gorden, Kimberly Sturk‐Andreaggi, Julia Warnke-Sommer, Amy Hazelwood et al.
STR artifacts are commonly observed in electrophoretic data and can complicate interpretation of the profiles produced. Even when a consensus approach is applied, reproducible artifacts have the potential to convolute the analysis. DNA obtained from historical bone samples is often heavily degraded ...
Katherine B. Gettings, Andreas Tillmar, Kimberly Sturk‐Andreaggi, Charla Marshall
Abstract In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan in place. Compared with traditional ...
Lindsay L Kotchey, Sophie Lee, Leah Nangeroni, Jacqueline Tyler Thomas et al.
Advancements in forensic science have introduced single nucleotide polymorphism (SNP) markers as crucial tools in kinship, ancestry, and identity testing, and in predicting phenotypic traits. The emergence of Forensic Genetic Genealogy (FGG) and massively parallel sequencing (MPS) technologies have ...
Andreas Tillmar, Kimberly Sturk‐Andreaggi, Adam Staadig, Christina Amory et al.
This study evaluated the ability to produce FORensic Capture Enrichment (FORCE) genotypes using amplicon-based and capture-based enrichment assays. The FORCE panel is a standardized set of single nucleotide polymorphism (SNP) markers developed for forensic applications. Twelve DNA samples were prepa...
Ciara N. Rhodes, Jacqueline Tyler Thomas, Kimberly Sturk‐Andreaggi, Tal Simmons et al.
Advances in DNA technology have significantly enhanced the forensic community's ability to develop genetic profiles from unidentified human skeletal remains. However, sampling requires mechanical grinding of hard tissues before DNA isolation. This processing can compromise genetic profiles, particul...