Firdausi Qadri, Rubhana Raqib, Firoz Ahmed, Taufiqur Rahman et al.
Investigations were carried out to study the production of factors associated with the innate immune response in the systemic and mucosal compartments in adults and children infected with Vibrio cholerae O1 and V. cholerae O139. The levels of nonspecific mediators of the innate defense system, i.e.,...
Claudia Rodas, Volga Iñiguez, Firdausi Qadri, Gudrun Wiklund et al.
ABSTRACT Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in...
Roberto Vidal, Khitam Muhsen, Sharon M. Tennant, Ann‐Mari Svennerholm et al.
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four ente...
John D. Clemens, F. van Loon, David A. Sack, J. Chakraborty et al.
The relationship of serum vibriocidal (VC) and IgG anti-cholera toxin (CT) antibodies to the risk of cholera was evaluated during the first year of follow-up of recipients of three oral doses of B subunit (BS)-whole-cell vaccine, whole-cell vaccine, or Escherichia coli K12 strain placebo in Banglade...
John D. Clemens, David A. Sack, Jeffrey R. Harris, M. R. Khan et al.
Journal Article BREAST FEEDING AND THE RISK OF SEVERE CHOLERA IN RURAL BANGLADESHI CHILDREN Get access JOHN D. CLEMENS, JOHN D. CLEMENS 1International Centre for Diarrhoeal Disease ResearchBangladesh, Dhaka, Bangladesh2Center for Vaccine Development, University of Maryland School of MedicineBaltimor...
Fahima Chowdhury, Mohammad Arif Rahman, Yasmin Ara Begum, Ashraful Islam Khan et al.
BACKGROUND: In Bangladesh, increases in cholera epidemics are being documented with a greater incidence and severity. The aim of this prospective study was to identify the prevalence and importance of V. cholerae O1 and enterotoxigenic Escherichia coli (ETEC) as causal agents of severe diarrhea in a...
Ingrid Bölin, Gudrun Wiklund, Firdausi Qadri, Olga Torres et al.
Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhea among children in developing countries and in travelers to areas of ETEC endemicity. ETEC strains isolated from humans may produce a heat-labile enterotoxin (LT) and two types of the heat-stable enterotoxin STa, called STh and...
Åsa Lothigius, Anders Janzon, Yasmin Ara Begum, Åsa Sjöling et al.
AIMS: We aimed to develop an assay for sensitive detection and quantification of enterotoxigenic Escherichia coli (ETEC) in different types of water samples. METHODS AND RESULTS: Real-time polymerase chain reaction (PCR) assays with primers against ETEC enterotoxin genes estA (STh) estB (STp) and el...
Yasmin Ara Begum, Kaisar A. Talukder, Ishrat J. Azmi, Mohammad Shahnaij et al.
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children as well as in travellers to ETEC endemic countries. Ciprofloxacin is a broad-spectrum antimicrobial agent nowadays used for the treatment of dia...
Jan Holmgren, Ann‐Mari Svennerholm, Marianne Jertborn, John D. Clemens et al.
During the last decade there has been a rapid progress in the development of new, much improved vaccines against cholera. These vaccines, which are given orally to stimulate the gut mucosal immune system, are based on either a combination of purified cholera toxin B (binding) subunit and killed chol...
Firdausi Qadri, Christine Wennerås, Firoz Ahmed, Muhammad Asaduzzaman et al.
We have compared the B cell responses evoked in Bangladeshi, adults (n=11, median age 25 years) and children (n=21, median age 4.5 years), 7 days after intake of each of two doses of an oral, inactivated enterotoxigenic Escherichia coli (ETEC) vaccine composed of formalin-killed ETEC strains express...
Firdausi Qadri, Tanvir Ahmed, Firoz Ahmed, R. Bradley Sack et al.
A phase II safety and immunogenicity study of an oral-formalin inactivated enterotoxigenic Escherichia coli (ETEC) vaccine containing six colonization factors (CFA/I, CS1, CS2, CS3, CS4, CS5) and 1mg of recombinant cholera toxin B subunit (the CF-BS-ETEC vaccine) was carried out in an urban slum of ...
Tanvir Ahmed, Ann‐Mari Svennerholm, Abdullah A. Tarique, Gazi Nurun Nahar Sultana et al.
The killed oral cholera vaccine Dukoral is recommended for adults and only children over 2 years of age, although cholera is seen frequently in younger children and there is an urgent need for a vaccine for them. Since decreased immunogenicity of oral vaccines in children in developing countries is ...
Firdausi Qadri, Tanveer Ahmed, Faroque Ahmed, Yasmin Ara Begum et al.
The oral-formalin inactivated whole cell enterotoxigenic Escherichia coli (ETEC) vaccine needs to be further tested in developing countries in order to determine the dose at which it will be safe and immunogenic for infants who are the target population for the vaccine. To determine the immunogenici...
Marjahan Akhtar, Mohiul Islam Chowdhury, Taufiqur Rahman Bhuiyan, Joanna Kaim et al.
The safety and immunogenicity of the second generation oral enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX, consisting of inactivated recombinant E. coli strains over-expressing the colonization factors (CFs) CFA/I, CS3, CS5 and CS6 and the heat labile toxoid LCTBA, were evaluated in Banglade...