Patricia B. Pavlinac, James A Platts-Mills, Jie Liu, Hannah E Atlas et al.
BACKGROUND: Bacterial pathogens cause substantial diarrhea morbidity and mortality among children living in endemic settings, yet antimicrobial treatment is only recommended for dysentery or suspected cholera. METHODS: AntiBiotics for Children with severe Diarrhea was a 7-country, placebo-controlled...
Patricia B. Pavlinac, James A Platts-Mills, Kirkby D. Tickell, Jie Liu et al.
BACKGROUND: Shigella is a leading cause of childhood diarrhea and target for vaccine development. Microbiologic and clinical case definitions are needed for pediatric field vaccine efficacy trials. METHODS: We compared characteristics of moderate to severe diarrhea (MSD) cases in the Global Enteric ...
Suporn Pholwat, Jie Liu, Suzanne Stroup, Jean Gratz et al.
UNLABELLED: Genotypic methods for drug susceptibility testing of Mycobacterium tuberculosis are desirable to speed the diagnosis and proper therapy of tuberculosis (TB). However, the numbers of genes and polymorphisms implicated in resistance have proliferated, challenging diagnostic design. We deve...
Scott K. Heysell, Shahriar Ahmed, Sara Sabrina Ferdous, Md. Siddiqur Rahman Khan et al.
BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) treatment in Bangladesh is empiric or based on qualitative drug-susceptibility testing (DST) by comparative growth in culture media with and without a single drug concentration. METHODS: Adult patients were enrolled throughout Bangladesh during t...
Arfatur Rahman, Sara Sabrina Ferdous, Shahriar Ahmed, S. M. Mazidur Rahman et al.
ABSTRACT Pyrazinamide (PZA) is a frontline antituberculosis (anti-TB) drug used in both first- and second-line treatment regimens. However, due to complex laboratory requirements, the PZA susceptibility test is rarely performed, leading to a scarcity of data on susceptibility to PZA. Bangladesh is a...
Suporn Foongladda, Sayera Banu, Suporn Pholwat, Jean Gratz et al.
BACKGROUND: Although phenotypic drug susceptibility testing (DST) is endorsed as the standard for second-line drug testing of Mycobacterium tuberculosis, it is slow and laborious. METHODS: We evaluated the accuracy of two faster, easier methodologies that provide results for multiple drugs: a genoty...
Jie Liu, Kate Lurain, Shihab U. Sobuz, Sharmin Begum et al.
Rotavirus genotyping is useful for surveillance purposes especially in areas where rotavirus vaccination has been or will be implemented. RT-PCR based molecular methods have been applied widely, but quantitative assays targeting a broad spectrum of genotypes have not been developed. Three real time ...
Monica McGrath, Jessica C. Seidman, Martha I. Nelson, D R Lang et al.
Background: Norovirus (NRV) is an important cause of gastroenteritis in children in developed countries. The genus NRV has extensive genetic diversity and is divided into at least two genogroups, I (GI) and II (GII) based on sequence analysis of genes encoding structural and nonstructural proteins. ...