Lynda Mottram, Gudrun Wiklund, Göran Larson, Firdausi Qadri et al.
Polymorphisms of the FUT2 gene alters glycan ABO(H) blood group and Lewis antigen expression (commonly known as non-secretor status) in the small intestinal mucosa. Whilst non-secretor status affects 20% of the population worldwide, it has been reported to be present in up to 40% of all Bangladeshis...
J. D. Clemens, David A. Sack, J. Chakraborty, Malla Rao et al.
In a field trial conducted in Bangladesh, ingestion of either B subunit-killed whole cell (BS-WC) or killed whole cell (WC) oral cholera vaccines by mothers was associated with a 47% reduction of the risk of cholera in their non-vaccinated children aged under 36 months. Because vaccine-induced breas...
Christina Åhrén, Leif Gothefors, Barbara J. Stoll, Mogjiborahman Salek et al.
Fecal Escherichia coli isolates from 196 patients with watery diarrhea and 68 healthy individuals (controls) were analyzed in Bangladesh immediately after isolation for the presence of colonization factor antigen (CFA) I or II (CFA/I or CFA/II, respectively) by a mannose-resistant hemagglutination (...
David A. Sack, Firdausi Qadri, Ann–Mari Svennerholm
Oral vaccines which are intended for global use do not necessarily induce the same immune responses in all children worldwide. In fact, several vaccines often induce less frequent and lower mean antibody responses in children in developing countries, suggesting that the vaccines may be less protecti...
Ann‐Mari Svennerholm, G J Strömberg, Jan Holmgren
Soluble hemagglutinin (HA) from an El Tor Vibrio cholerae strain (serotype Ogawa) was purified by means of a sequence of salt precipitation, gel filtration, and agarose electrophoresis. The purified material, which gave a single precipitation line in immunodiffusion tests with homologous antiserum, ...
Sohel Shamsuzzaman, Tanvir Ahmed, Kaiissar Mannoor, Yasmin Ara Begum et al.
The emergence of V. cholerae O139 serogroup of V. cholerae capable of causing severe dehydrating cholera has over the decade led to efforts in formulation of vaccines to protect against this pathogen. Although the prevalence of diarrhea due to V. cholerae O139 has recorded a decrease, efforts on vac...
Amit Saha, Mohiul Islam Chowdhury, Mohammad Nazim, Mohammad Murshid Alam et al.
Background Immune responses to the inactivated oral whole cell cholera toxin B (CTB) subunit cholera vaccine, Dukoral(®), as well as three childhood vaccines in the national immunization system were compared in children living in high and low arsenic contaminated areas in Bangladesh. In addition, se...
Mohammad Murshid Alam, Amena Aktar, Sadia Afrin, Mohammad Arif Rahman et al.
BACKGROUND: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still unknown. Therefore, memory B cell responses and affinity maturation of antibodies ...
Firdausi Qadri, Tanvir Ahmed, Firoz Ahmed, Md Saruar Bhuiyan et al.
Colonization factor CS6 expressed by enterotoxigenic Escherichia coli (ETEC) is a nonfimbrial polymeric protein. A substantial proportion of ETEC strains isolated from patients in endemic settings and in people who travel to regions where ETEC is endemic are ETEC strains expressing CS6, either alone...
Taufiqur Rahman Bhuiyan, Feroza K. Choudhury, Farhana Khanam, Amit Saha et al.
Young children are very susceptible to typhoid fever, emphasizing the need for vaccination in under five age groups. The parenteral Vi polysaccharide vaccine is not immunogenic in children under 2 years and the oral Ty21a vaccine (Vivotif) available in capsular formulation is only recommended for th...
Firdausi Qadri, Firoz Ahmed, Tanvir Ahmed, Ann‐Mari Svennerholm
We have studied homologous (HoM) and cross-reacting (CR) immunoglobulin A (IgA) antibody responses to colonization factors (CFs) in Bangladeshi children with diarrhea due to enterotoxigenic E. coli (ETEC) strains of the CF antigen I (CFA/I) group (CFA/I, n = 25; coli surface antigen 4 [CS4], n = 8; ...
Åsa Sjöling, Firdausi Qadri, Matilda Nicklasson, Yasmin Ara Begum et al.
Enterotoxigenic Escherichia coli (ETEC) colonize the intestine and adhere to the epithelium by means of different host specific colonization factors (CFs). Colonizing ETEC produce one or both of two enterotoxins; the heat stable (ST) and heat labile (LT) toxins which are both able to cause diarrhoea...
M. Ansaruzzaman, N. A. Bhuiyan, Yasmin Ara Begum, Ingolf Kühn et al.
A total of 99 isolates out of 370 colonization factor (CF)-positive, well-characterized enterotoxigenic Escherichia coli (ETEC) strains belonging to 13 different CF types isolated from diarrhoeal patients admitted to the hospital of the International Centre for Diarrhoeal Disease Research, Banglades...
Fahima Chowdhury, Yasmin Ara Begum, Mohammad Murshid Alam, Ashraful Islam Khan et al.
Vibrio cholerae O1 and enterotoxigenic Escherichia coli (ETEC) are major bacterial pathogens that cause dehydrating disease requiring hospitalization of children and adults. The cholera toxin (CT) produced by V. cholerae O1 and the heat-labile toxin (LT) and/or heat-stable toxin (ST) of ETEC are res...
Stephen R. Attridge, Camilla Johansson, Dang Duc Trach, Firdausi Qadri et al.
A microplate assay for the detection of bactericidal antibodies to Vibrio cholerae O139 is described. The assay is sensitive, highly reproducible, specific, and convenient to perform. It has been used to demonstrate the induction of serum bactericidal antibodies in Vietnamese recipients of an oral, ...
Ann‐Mari Svennerholm, Anna Lundgren, Susannah Leach, Marjahan Akhtar et al.
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of mortality and morbidity in children in low-income countries. We have tested an oral ETEC vaccine, ETVAX, consisting of inactivated E coli overexpressing the most prevalent colonization factors and a toxoid, LCTBA, administered together wi...
Firdausi Qadri, Ann–Mari Svennerholm, Sohel Shamsuzzaman, Taufiqur Rahman Bhuiyan et al.
Vibrio cholerae O139 emerged in 1992 as a major cause of epidemic cholera. However, the incidence of disease due to this new serogroup subsequently decreased for almost a decade. In April 2002, there was a dramatic resurgence of V. cholerae O139 in Bangladesh. We compared the phenotypic properties o...
Marjahan Akhtar, Firdausi Qadri, Taufiqur Rahman Bhuiyan, Sarmin Akter et al.
Immune responses to oral enteric vaccines in children and infants may be influenced by factors such as age, previous priming with related microorganisms and breast feeding. In this study, we aimed to determine optimal time points to assess immune responses to oral enteric vaccines in different clini...
Ann‐Mari Svennerholm, Firdausi Qadri, Anna Lundgren, Joanna Kaim et al.
We tested an oral enterotoxigenic Escherichia coli (ETEC) vaccine, ETVAX, consisting of inactivated E. coli overexpressing the most prevalent ETEC colonization factors (CFs) and a toxoid (LCTBA), in Bangladeshi children for capacity to induce mucosal and plasma immune responses against O78 lipopolys...
Sachin Mani, Franklin R. Toapanta, Monica A. McArthur, Firdausi Qadri et al.
The generation of robust systemic and mucosal antibody and cell-mediated immune (CMI) responses that are protective, long-lasting, and can quickly be recalled upon subsequent re-exposure to the cognate antigen is the key to the development of effective vaccine candidates. These responses, whether th...