Cynthia L. Sears, Salequl Islam, Amit Saha, Maleka Arjumand et al.
BACKGROUND: Diarrheal illnesses remain a leading cause of morbidity and mortality globally, with increasing recognition of long-term sequelae, including postinfectious irritable bowel syndrome and growth faltering, as well as cognitive deficits in children. Identification of specific etiologic agent...
Hadi Abd, Amir Saeed, Andrej Weintraub, G. Balakrish Nair et al.
Vibrio cholerae species are extracellular, waterborne, gram-negative bacteria that are overwhelmed by predators in aquatic environments. The unencapsulated serogroup V. cholerae O1 and encapsulated V. cholerae O139 cause epidemic and pandemic outbreaks of cholera. It has recently been shown that the...
Shah M. Faruque, Nityananda Chowdhury, Muhammad Kamruzzaman, Qazi Shafi Ahmad et al.
During March and April 2002, a resurgence of Vibrio cholerae O139 occurred in Dhaka and adjoining areas of Bangladesh with an estimated 30,000 cases of cholera. Patients infected with O139 strains were much older than those infected with O1 strains (p<0.001). The reemerged O139 strains belong to a s...
Yuriy A. Knirel, Liliana Paredes, Per‐Erik Jansson, Andrej Weintraub et al.
The capsular polysaccharide (CPS) of Vibrio cholerae O139 synonym Bengal, which is thought to carry determinants of O-specificity, was isolated by phenol/water extraction followed by delipidation of the contaminating lipopolysaccharide at pH 4.2 and gel-permeation chromatography. The CPS contained D...
Andrej Weintraub, Göran Widmalm, Per‐Erik Jansson, Monica Jansson et al.
A newly described Vibrio cholerae serogroup--O139 Bengal, the causative agent of the recent large epidemics of cholera-like disease in the Indian subcontinent and neighbouring countries--possesses a high molecular weight capsular polysaccharide (CPS) that can be visualized by electron microscopy and...
M. John Albert, Dr. Md. Shahidul Islam, Shamsun Nahar, Firdausi Qadri et al.
In a previous study using pure bacterial cultures in a PCR assay, a primer pair corresponding to a unique chromosomal region of Vibrio cholerae O139 Bengal generated an amplicon from only V. cholerae O139 Bengal. PCR with the same primer pair was used to screen 180 diarrheal stool specimens. All the...
M. John Albert, N. A. Bhuiyan, A Rahman, Avishek Ghosh et al.
From the stool of a Vibrio cholerae O139 Bengal-infected patient, a phage that specifically lysed capsulated V. cholerae O139 strains only was isolated. The phage is useful for the confirmatory diagnosis of V. cholerae O139 infection and for the differentiation of variants that lack the capsule.
Reine Eserstam, Thushari P. Rajaguru, Per‐Erik Jansson, Andrej Weintraub et al.
The structure of the O-polysaccharide of the lipopolysaccharide from a diarrheal strain isolated in Bangladesh was studied with sugar, and methylation analysis, NMR spectroscopy, mass spectrometry and partial acid hydrolysis. The strain was first designated as Hafnia alvei, but later found to be a p...
Firdausi Qadri, Ann–Mari Svennerholm, Sohel Shamsuzzaman, Taufiqur Rahman Bhuiyan et al.
Vibrio cholerae O139 emerged in 1992 as a major cause of epidemic cholera. However, the incidence of disease due to this new serogroup subsequently decreased for almost a decade. In April 2002, there was a dramatic resurgence of V. cholerae O139 in Bangladesh. We compared the phenotypic properties o...
Malin Linnerborg, Göran Widmalm, Andrej Weintraub, M. John Albert
Sugar and methylation analyses of native polysaccharides together with one-dimensional 1H- and 13C-NMR spectroscopy revealed that the two polysaccharides from strains 22074 and 12254 of Plesiomonas shigelloides are identical. The structure of the polysaccharide from strain 22074 was deduced from a u...
Malin Linnerborg, Andrej Weintraub, M. John Albert, Göran Widmalm
We have studied the interaction between the Vibrio cholerae O139 specific phage JA1, belonging to the Podoviridae family, and the capsular polysaccharide (CPS) of the parent strain from which the phage was isolated. Upon incubation of the JA1 phage with the CPS, oligosaccharides were isolated and pu...
Susanna Falklind-Jerkérus, Franco Felici, Cristina Cavalieri, Carla Lo Passo et al.
Vibrio cholerae is the etiological agent of cholera. V. cholerae serogroup O1 had been, until 1992, the only serogroup responsible for large epidemics and pandemics of cholera. In 1992, a new serotype of V. cholerae emerged in South-East Asia that caused a massive outbreak of cholera in India and ne...
Elin Säwén, Jennie Östervall, Clas Landersjö, Malin Edblad et al.
The structure of the repeating unit of the O-antigenic polysaccharide from Plesiomonas shigelloides strain AM36565 has been determined. Component analysis and (1)H and (13)C NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by (1)H,(13)...
Yuriy A. Knirel, Sof’ya N. Senchenkova, Per‐Erik Jansson, Andrej Weintraub et al.
The O-specific polysaccharide of an Aeromonas trota strain was isolated by hydrolysis of the lipopolysaccharide at pH 4.5 followed by gel-permeation chromatography and found to consist of hexasaccharide repeating units containing D-galactose, L-rhamnose, 3,6-dideoxy-L-xylo-hexose (colitose, Col), 2-...
Carolina Fontana, Mona Zaccheus, Andrej Weintraub, Mohammad Ansaruzzaman et al.
The structure of a polysaccharide from Vibrio parahaemolyticus strain AN-16000 has been investigated. The sugar and absolute configuration analysis revealed d-Glc, d-GalN, d-QuiN and l-FucN as major components. The PS was subjected to dephosphorylation with aqueous 40% HF to obtain an oligosaccharid...
M. John Albert, Firdausi Qadri, Nurul A. Bhuiyan, Shaikh Meshbahuddin Ahmad et al.
Capsulated bacteria exhibit serum (complement) resistance and resistance to phagocytosis, which result in disseminated infections. Vibrio cholerae O139 strains possess a thin capsule and have been found to be partially serum resistant in a previous study. In the present study, compared to a standard...
Clas Landersjö, Andrej Weintraub, M. Ansaruzzaman, M. John Albert et al.
The O-antigenic polysaccharide part of the lipopolysaccharide from Vibrio mimicus N-1990 has been investigated. Sugar and methylation analysis of native and dephosphorylated polysaccharide together with NMR spectroscopy show that the polysaccharide is composed of tetrasaccharide repeating units. The...
Alexandra Kjellberg, Andrej Weintraub, M. John Albert, Göran Widmalm
The structure of the O-antigenic polysaccharide part of the lipopolysaccharide isolated from Vibrio cholerae O10 has been determined. The main method used has been 1H- and 13C-NMR spectroscopy. Sugar and methylation analyses were also applied to the polysaccharide. The tetrasaccharide repeating unit...
Sof’ya N. Senchenkova, George V. Zatonsky, Alexander S. Shashkov, Yuriy A. Knirel et al.
The O-specific polysaccharide of Vibrio cholerae 0155 was studied by sugar and methylation analyses, dephosphorylation with 48% hydrofluoric acid, 1H- and 13C-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and heteronuclear single-quantum coherence (HSQC) experiments. The following ...
Susanna Falklind-Jerkérus, M. John Albert, Andrej Weintraub
Vibrio cholerae O139 Bengal emerged as a second aetiologic agent of cholera in South Asia in late 1992. This new serogroup arose from a Vibrio cholerae O1 strain by deletion of the chromosomal region encoding O1 specificity and acquisition of a novel 35-kb region encoding the O139 specificity. Previ...