Naïma Madi, Emilee Cato, Md. Abu Sayeed, Ashton Creasy-Marrazzo et al.
Despite an increasingly detailed picture of the molecular mechanisms of bacteriophage (phage)–bacterial interactions, we lack an understanding of how these interactions evolve and impact disease within patients. In this work, we report a year-long, nationwide study of diarrheal disease patients in B...
Ashton Creasy-Marrazzo, Morteza M. Saber, Manasi Kamat, Laura S. Bailey et al.
The antibiotic formulary is threatened by high rates of antimicrobial resistance (AMR) among enteropathogens. Enteric bacteria are exposed to anaerobic conditions within the gastrointestinal tract, yet little is known about how oxygen exposure influences AMR. The facultative anaerobe Vibrio cholerae...
Naïma Madi, Emilee Cato, Md. Abu Sayeed, Ashton Creasy-Marrazzo et al.
Abstract Despite an increasingly detailed picture of the molecular mechanisms of phage-bacterial interactions, we lack an understanding of how these interactions evolve and impact disease within patients. Here we report a year-long, nation-wide study of diarrheal disease patients in Bangladesh. Amon...
Chuhan Qin, Patrick Lypaczewski, Md. Abu Sayeed, Aline Cuénod et al.
Cholera is an acute diarrhoeal disease caused by Vibrio cholerae . It remains a major public health challenge worldwide, and particularly in the endemic region around the Bay of Bengal. Over decadal time scales, one lineage typically dominates and spreads in global pandemic waves. However, it remain...
S. M. Ahmed, Md. Abu Sayeed, Ishae Sriguha, Emilee Cato et al.
ABSTRACT Background Effective cholera outbreak response requires accurate bedside rapid diagnostic tests (RDTs) because access to laboratories is often limited. Formative studies suggest cholera diagnostics have multiple vulnerabilities, including antibiotics and predation by bacteriophage (‘phage’)...
Chuhan Qin, Patrick Lypaczewski, Md. Abu Sayeed, Aline Cuénod et al.
Abstract Cholera is a diarrhoeal disease caused by Vibrio cholerae . It remains a major public health challenge in the endemic region around the Bay of Bengal. Over decadal time scales, one lineage typically dominates the others and spreads in global pandemic waves. However, it remains unclear to wh...
Ishae Sriguha, Michelle Mu, Md. Abu Sayeed, Emilee Cato et al.
ABSTRACT Molecular diagnostics to detect Vibrio cholerae ( Vc ) may be negatively impacted by pathogen-specific lytic bacteriophage (phage) predation. To address this problem, phage detection as a proxy for pathogen detection has been proposed. However, efforts to modernize cholera diagnostics with ...
Naïma Madi, Md. Abu Sayeed, Emilee Cato, Ashton Creasy-Marrazzo et al.
Phage predation is inversely associated with severe cholera yet its importance as a determinant of dehydration severity is unknown relative to other factors. Here we used machine learning to assess and rank potential host, microbial, and environmental factors as determinants of severe dehydration am...
Ashton Creasy-Marrazzo, Magdy Saber, Manasi Kamat, Laura S. Bailey et al.
Abstract The antibiotic formulary is threatened by high rates of antimicrobial resistance (AMR) among enteropathogens. Enteric bacteria are exposed to anaerobic conditions within the gastrointestinal tract, yet little is known about how oxygen exposure influences AMR. The facultative anaerobe Vibrio...