Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman et al.
BACKGROUND: Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analysis. RESULTS: To overcome this limitation, we develop SCoPE...
Aleksandra A. Petelski, Edward Emmott, Andrew Leduc, R. Gray Huffman et al.
Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either label-free or multiplexed) have enabled quantifyi...
Laurent Gatto, Ruedi Aebersold, Jüergen Cox, Vadim Demichev et al.
A community of researchers working in the emerging field of single-cell proteomics propose best-practice experimental and computational recommendations and reporting guidelines for studies analyzing proteins from single cells by mass spectrometry. Analyzing proteins from single cells by tandem mass ...
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia et al.
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed prioritized Single-Cell ProtEomics (pSCoPE). pSCoPE...
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia et al.
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth of protein quantification, especially for proteins and modifications of biological interest. To simultaneously advance all these aims, we developed prioritized Single Cell ProtEomics (pSCoPE). pSCoPE cons...
James Horncastle
AbstractThe limited studies that focus on the weaponization of refugees typically emphasize how liberal democracies and states with restricted carrying capacity are vulnerable to this tactic. The declining number of liberal democracies globally, the Russian-Ukraine War demonstrating states' true car...
Myra Hosmillo, Jia Lu, Michael R. McAllaster, James B. Eaglesham et al.
Abstract Knowledge of the host factors required for norovirus replication has been hindered by the challenges associated with culturing human noroviruses. We have combined proteomic analysis of the viral translation and replication complexes with a CRISPR screen, to identify host factors required fo...
Myra Hosmillo, Jia Lu, Michael R. McAllaster, James B. Eaglesham et al.
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Knowledge of the host factors required for norovirus replication has been hindered by the ...
S Weinberg, Alex Horsley, Eva Caamaño‐Gutiérrez, Michael M. Tunney et al.
S Weinberg, Alex Horsley, Eva Caamaño‐Gutiérrez, Michael M. Tunney et al.