Harrison Specht, Edward Emmott, Aleksandra A. Petelski, R. Gray Huffman et al.
BACKGROUND: Macrophages are innate immune cells with diverse functional and molecular phenotypes. This diversity is largely unexplored at the level of single-cell proteomes because of the limitations of quantitative single-cell protein analysis. RESULTS: To overcome this limitation, we develop SCoPE...
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman et al.
Proteomics of small sample sizes using data-independent acquisition methods achieves higher throughput with multiplexing. Current mass spectrometry methods enable high-throughput proteomics of large sample amounts, but proteomics of low sample amounts remains limited in depth and throughput. To incr...
Aleksandra A. Petelski, Edward Emmott, Andrew Leduc, R. Gray Huffman et al.
Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either label-free or multiplexed) have enabled quantifyi...
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia et al.
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth of protein quantification, especially for proteins and modifications of biological interest. Here, to simultaneously advance all these aims, we developed prioritized Single-Cell ProtEomics (pSCoPE). pSCoPE...
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman et al.
Current mass-spectrometry methods enable high-throughput proteomics of large sample amounts, but proteomics of low sample amounts remains limited in depth and throughput. To increase the throughput of sensitive proteomics, we developed an experimental and computational framework, plexDIA, for simult...
R. Gray Huffman, Andrew Leduc, Christoph Wichmann, Marco Di Gioia et al.
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth of protein quantification, especially for proteins and modifications of biological interest. To simultaneously advance all these aims, we developed prioritized Single Cell ProtEomics (pSCoPE). pSCoPE cons...
Aleksandra A. Petelski, Nikolai Slavov, Harrison Specht
Single-cell proteomics analysis requires sensitive, quantitatively accurate, widely accessible, and robust methods. To meet these requirements, the Single-Cell ProtEomics (SCoPE2) protocol was developed as a second-generation method for quantifying hundreds to thousands of proteins from limited samp...
Lisa K. Iwamoto-Stohl, Aleksandra A. Petelski, Baiyi Quan, Maciej Meglicki et al.
While non-mammalian embryos often rely on spatial pre-patterning, mammalian development has long been thought to begin with equivalent blastomeres. However, emerging evidence challenges this. Here, using multiplexed and label-free single-cell proteomics, we identify over 300 asymmetrically abundant ...
Harrison Specht, Maddy Yeh, Sarah N. Sipe, David Barnes‐Seeman et al.
Mass spectrometry-based proteomics enables comprehensive characterization of protein abundance, function, and interactions. Label-free approaches are simple to implement but challenging to scale to thousands of samples per day. Multiplexed techniques, such as plexDIA, can address these limitations b...
Aleksandra A. Petelski, Nikolai Slavov, Harrison Specht
Single-cell proteomics analysis requires sensitive, quantitatively accurate, widely accessible, and robust methods. To meet these requirements, the Single-Cell ProtEomics (SCoPE2) protocol was developed as a second-generation method for quantifying hundreds to thousands of proteins from limited samp...