A.‐M. Svennerholm, Marianne Jertborn, I. e. Gothefors, Amr M. Karim et al.
Mucosal and systemic immune responses to a new oral cholera vaccine, consisting of the B subunit plus killed vibrios, were studied in Bangladeshi volunteers and compared with those to clinical cholera. A single peroral dose of vaccine induced a local IgA antitoxin response in intestinal-lavage fluid...
Leif Gothefors, C Ahrén, Barbara J. Stoll, D Barua et al.
In Dhaka, Bangladesh, fresh isolates of Escherichia coli from 197 patients with diarrhea were investigated for production of enterotoxin and possession of colonization factor antigen (CFA) I or II. Enterotoxigenic E. coli (ETEC) was isolated from 34% of the patients, and of the 67 enterotoxin-positi...
Barbara J. Stoll, A.‐M. Svennerholm, Leif Gothefors, D Barua et al.
Fifteen patients hospitalized with acute, watery diarrhea and with enterotoxigenic Escherichia coli (ETEC) detected from stool samples were studied to evaluate the extent to which natural ETEC diarrhea induces local and systemic antibody responses to E. coli heat-labile toxin (LT), homologous lipopo...
Ann‐Mari Svennerholm, Leif Gothefors, David A. Sack, P K Bardhan et al.
A single oral or intramuscular immunization with purified cholera B subunit induced an intestinal secretory immunoglobulin A (IgA) antitoxin response in, respectively, 10 out of 11 and 9 out of 12 Bangladeshi volunteers. The IgA titre rise in intestinal lavage fluid was similar by either route of im...
Christina Åhrén, Leif Gothefors, Barbara J. Stoll, Mogjiborahman Salek et al.
Fecal Escherichia coli isolates from 196 patients with watery diarrhea and 68 healthy individuals (controls) were analyzed in Bangladesh immediately after isolation for the presence of colonization factor antigen (CFA) I or II (CFA/I or CFA/II, respectively) by a mannose-resistant hemagglutination (...
Jan Holmgren, A.‐M. Svennerholm, Leif Gothefors, Marianne Jertborn et al.
We examined whether infection with enterotoxigenic Escherichia coli (ETEC) producing the heat-labile enterotoxin (LT) can prime the gut immune system to respond more efficiently to the immunologically related cholera B subunit component of a recently developed oral B subunit-whole-cell cholera vacci...
A.‐M. Svennerholm, Marianne Jertborn, Leif Gothefors, A. Karim et al.
Purified B subunit of cholera toxin retains membrane-binding capacity and protective immunogenicity and yet has no toxic activity as tested in animals. These properties suggest that B subunit might be a promising immunogen, particularly as an oral vaccine, for stimulating protective antitoxic immuni...
Nigar S. Shahid, A N Alam, K Haider, C Henning et al.
Tracheal aspirates from patients with diarrhoea and pneumonia were tested for the presence of pneumococci by both quantitative culture and counter immunoelectrophoresis (CIE). Antigen was demonstrated by CIE in 33/48 of these confirmed cases of pneumonia. The technique is easy to use and gives rapid...