Karine Brudey, Jeffrey Driscoll, Leen Rigouts, Wolfgang M. Prodinger et al.
BACKGROUND: The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member of the CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping is the widely used PCR-based reverse-hybridization blotting technique that assays the genetic dive...
Chen-Shan Chin, Jon M. Sorenson, Jason B. Harris, William P. Robins et al.
BACKGROUND: Although cholera has been present in Latin America since 1991, it had not been epidemic in Haiti for at least 100 years. Recently, however, there has been a severe outbreak of cholera in Haiti. METHODS: We used third-generation single-molecule real-time DNA sequencing to determine the ge...
Jason B Harris, Regina C LaRocque, Firdausi Qadri, Edward T Ryan et al.
Cholera is an acute, secretory diarrhoea caused by infection with Vibrio cholerae of the O1 or O139 serogroup. It is endemic in more than 50 countries and also causes large epidemics. Since 1817, seven cholera pandemics have spread from Asia to much of the world. The seventh pandemic began in 1961 a...
Shannon Takala‐Harrison, Christopher G. Jacob, Cesar Arze, Michael P. Cummings et al.
BACKGROUND: The emergence of artemisinin-resistant Plasmodium falciparum in Southeast Asia threatens malaria treatment efficacy. Mutations in a kelch protein encoded on P. falciparum chromosome 13 (K13) have been associated with resistance in vitro and in field samples from Cambodia. METHODS: P. fal...
Syed Ahsanuddin Ahmed, Motunrayo Ajisola, Kehkashan Azeem, Pauline Bakibinga et al.
INTRODUCTION: With COVID-19, there is urgency for policymakers to understand and respond to the health needs of slum communities. Lockdowns for pandemic control have health, social and economic consequences. We consider access to healthcare before and during COVID-19 with those working and living in...
Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman et al.
Proteomics of small sample sizes using data-independent acquisition methods achieves higher throughput with multiplexing. Current mass spectrometry methods enable high-throughput proteomics of large sample amounts, but proteomics of low sample amounts remains limited in depth and throughput. To incr...
Jason B. Harris, Regina C. LaRocque, Fahima Chowdhury, Ashraful Islam Khan et al.
BACKGROUND: Despite recent progress in understanding the molecular basis of Vibrio cholerae pathogenesis, there is relatively little knowledge of the factors that determine the variability in human susceptibility to V. cholerae infection. METHODS AND FINDINGS: We performed an observational study of ...
Brian Schwartz, Jason B. Harris, Ashraful Islam Khan, Regina C. LaRocque et al.
We examined demographic, microbiologic, and clinical data from patients presenting during 1988, 1998, and 2004 flood-associated diarrheal epidemics at a diarrhea treatment hospital in Dhaka, Bangladesh. Compared with non-flood periods, individuals presenting during flood-associated epidemics were ol...
Jason B. Harris, Ashraful Islam Khan, Regina C. LaRocque, David J. Dorer et al.
Individuals with blood group O are more susceptible than other individuals to severe cholera, although the mechanism underlying this association is unknown. To assess the respective roles of both intrinsic host factors and adaptive immune responses that might influence susceptibility to infection wi...
Lawrence A. David, Ana A. Weil, Edward T. Ryan, Stephen B. Calderwood et al.
UNLABELLED: Disability after childhood diarrhea is an important burden on global productivity. Recent studies suggest that gut bacterial communities influence how humans recover from infectious diarrhea, but we still lack extensive data and mechanistic hypotheses for how these bacterial communities ...
Ana A. Weil, Ashraful Islam Khan, Fahima Chowdhury, Regina C. LaRocque et al.
BACKGROUND: Multiple Vibrio cholerae infections in the same household are common. The objective of this study was to examine the incidence of V. cholerae infection and associated clinical symptoms in household contacts of patients with cholera and to identify risk factors for development of severe d...
Paul Farmer, Charles Patrick Almazor, Emily T. Bahnsen, Donna Barry et al.
Meeting Cholera's Challenge to Haiti and the World: A Joint Statement on Cholera Prevention and Care
Kimberley D. Seed, Minmin Yen, B. Jesse Shapiro, Isabelle J. Hilaire et al.
The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen d...
Aaron M. Harris, Fahima Chowdhury, Yasmin Ara Begum, Ashraful Islam Khan et al.
Bangladesh experienced severe flooding and diarrheal epidemics in 2007. We compared flood data from 2007 with 2004 and 1998 for diarrheal patients attending the ICDDR,B hospital in Dhaka. In 2007, Vibrio cholerae O1 (33%), rotavirus (12%), and enterotoxigenic Escherichia coli (ETEC) (12%) were most ...
Aaron M. Harris, Md Saruar Bhuiyan, Fahima Chowdhury, Ashraful Islam Khan et al.
Cholera, caused by Vibrio cholerae, is a noninvasive dehydrating enteric disease with a high mortality rate if untreated. Infection with V. cholerae elicits long-term protection against subsequent disease in countries where the disease is endemic. Although the mechanism of this protective immunity i...