Elizabeth A. Ashley, Mehul Dhorda, Rick M. Fairhurst, Chanaki Amaratunga et al.
BACKGROUND: Artemisinin resistance in Plasmodium falciparum has emerged in Southeast Asia and now poses a threat to the control and elimination of malaria. Mapping the geographic extent of resistance is essential for planning containment and elimination strategies. METHODS: Between May 2011 and Apri...
Sivaramakrishnan Rajaraman, Sameer Antani, Mahdieh Poostchi, Kamolrat Silamut et al.
parasites transmitted through the bite of female Anopheles mosquito. Microscopists commonly examine thick and thin blood smears to diagnose disease and compute parasitemia. However, their accuracy depends on smear quality and expertise in classifying and counting parasitized and uninfected cells. Su...
Zhaohui Liang, Andrew J. Powell, Ilker Ersoy, Mahdieh Poostchi et al.
Malaria is a major global health threat. The standard way of diagnosing malaria is by visually examining blood smears for parasite-infected red blood cells under the microscope by qualified technicians. This method is inefficient and the diagnosis depends on the experience and the knowledge of the p...
Daniel H. Paris, Mallika Imwong, Abul Faiz, Mahtabuddin Hasan et al.
A recently described loop-mediated isothermal polymerase chain reaction (LAMP) for molecular detection of Plasmodium falciparum was compared with microscopy, PfHRP2-based rapid diagnostic test (RDT), and nested polymerase chain reaction (PCR) as the "gold standard" in 115 Bangladeshi in-patients wit...
Katherine Plewes, Hugh W. F. Kingston, Aniruddha Ghose, Richard J. Maude et al.
Intravascular hemolysis is an intrinsic feature of severe malaria pathophysiology but the pathogenic role of cell-free hemoglobin-mediated oxidative stress in severe malaria associated acute kidney injury (AKI) is unknown. As part of a prospective observational study, enrolment plasma cell-free hemo...
Sivaramakrishnan Rajaraman, Kamolrat Silamut, Md. Aiub Hossain, Ilker Ersoy et al.
Convolutional neural networks (CNNs) have become the architecture of choice for visual recognition tasks. However, these models are perceived as black boxes since there is a lack of understanding of the learned behavior from the underlying task of interest. This lack of transparency is a serious dra...
Katherine Plewes, Hugh W. F. Kingston, Aniruddha Ghose, Thanaporn Wattanakul et al.
Background: Acute kidney injury independently predicts mortality in falciparum malaria. It is unknown whether acetaminophen's capacity to inhibit plasma hemoglobin-mediated oxidation is renoprotective in severe malaria. Methods: This phase 2, open-label, randomized controlled trial conducted at two ...
Richard J. Maude, Frederik Barkhof, Mahtab Uddin Hassan, Aniruddha Ghose et al.
BACKGROUND: Magnetic resonance imaging (MRI) allows detailed study of structural and functional changes in the brain in patients with cerebral malaria. METHODS: In a prospective observational study in adult Bangladeshi patients with severe falciparum malaria, MRI findings in the brain were correlate...
Richard J. Maude, Kamolrat Silamut, Katherine Plewes, Prakaykaew Charunwatthana et al.
BACKGROUND: Cytoadherence and sequestration of erythrocytes containing mature stages of Plasmodium falciparum are central to the pathogenesis of severe malaria. The oral anthelminthic drug levamisole inhibits cytoadherence in vitro and reduces sequestration of late-stage parasites in uncomplicated f...
Michael Herdman, Natthida Sriboonvorakul, Stije J. Leopold, Sam Douthwaite et al.
INTRODUCTION: Severe falciparum malaria is commonly complicated by metabolic acidosis. Together with lactic acid (LA), other previously unmeasured acids have been implicated in the pathogenesis of falciparum malaria. METHODS: In this prospective study, we characterised organic acids in adults with s...
Golnaz Moallem, Mahdieh Poostchi, Hang Yu, Kamolrat Silamut et al.
A malarial infection is diagnosed and monitored by screening microscope images of blood smears for parasite-infected red blood cells. Millions of blood slides are manually screened for parasites every year, which is a tedious and error-prone process, and which largely depends on the expertise of the...
Golnaz Moallem, Hamed Sari‐Sarraf, Mahdieh Poostchi, Richard J. Maude et al.
Automated image analysis of slides of thin blood smears can assist with early diagnosis of many diseases. Automated detection and segmentation of red blood cells (RBCs) are prerequisites for any subsequent quantitative highthroughput screening analysis since the manual characterization of the cells ...
Sivaramakrishnan Rajaraman, Sameer Antani, Mahdieh Poostchi, Kamolrat Silamut et al.
Malaria is a blood disease caused by the Plasmodium parasites transmitted through the bite of female Anopheles mosquito.Microscopists commonly examine thick and thin blood smears to diagnose disease and compute parasitemia.However, their accuracy depends on smear quality and expertise in classifying...