Karine Brudey, Jeffrey Driscoll, Leen Rigouts, Wolfgang M. Prodinger et al.
BACKGROUND: The Direct Repeat locus of the Mycobacterium tuberculosis complex (MTC) is a member of the CRISPR (Clustered regularly interspaced short palindromic repeats) sequences family. Spoligotyping is the widely used PCR-based reverse-hybridization blotting technique that assays the genetic dive...
Armand Van Deun, K. J. M. Aung, Valentin Bola, Rossin Lebeke et al.
The rapid diagnosis of rifampin resistance is hampered by a reported insufficient specificity of molecular techniques for detection of rpoB mutations. Our objective for this study was to document the prevalence and prognostic value of rpoB mutations with unclear phenotypic resistance. The study desi...
Matteo Zignol, Anna Dean, Natavan Alikhanova, Sönke Andres et al.
BACKGROUND: Pyrazinamide and fluoroquinolones are essential antituberculosis drugs in new rifampicin-sparing regimens. However, little information about the extent of resistance to these drugs at the population level is available. METHODS: In a molecular epidemiology analysis, we used population-bas...
Matteo Zignol, Andrea Maurizio Cabibbe, Anna Dean, Philippe Glaziou et al.
BACKGROUND: In many countries, regular monitoring of the emergence of resistance to anti-tuberculosis drugs is hampered by the limitations of phenotypic testing for drug susceptibility. We therefore evaluated the use of genetic sequencing for surveillance of drug resistance in tuberculosis. METHODS:...
Armand Van Deun, K. J. M. Aung, Md Anwar Hossain, Pim de Rijk et al.
SETTING: Greater Mymensingh area, Bangladesh. OBJECTIVES: To document among new tuberculosis (TB) patients the proportions and treatment outcomes of silent, non-disputed and disputed (generally missed by rapid drug susceptibility testing [DST]) rpoB mutations, and their detection by commercial molec...
Isdore Chola Shamputa, Leen Rigouts, Lovet Achale Eyongeta, Nabil Abdullah El Aila et al.
Although the heterogeneity of Mycobacterium tuberculosis populations and the existence of mixed infections are now generally accepted, systematic studies on their relative importance are rare. In the present study, 10 individual colonies of each M. tuberculosis isolate (primary isolate) from 97 tube...
Leen Rigouts, Nele Coeck, Mourad Gumusboga, Willem Bram de Rijk et al.
OBJECTIVES: Mutations in the gyrase genes cause fluoroquinolone resistance in Mycobacterium tuberculosis. However, the predictive value of these markers for clinical outcomes in patients with MDR-TB is unknown to date. The objective of this study was to determine molecular markers and breakpoints pr...
Simone Battaglia, Andrea Spitaleri, Andrea Maurizio Cabibbe, Conor J. Meehan et al.
The role of mutations in genes associated with phenotypic resistance to bedaquiline (BDQ) and delamanid (DLM) in Mycobacterium tuberculosis complex (MTBc) strains is poorly characterized. A clear understanding of the genetic variants’ role is crucial to guide the development of molecular-based drug ...
Armand Van Deun, K. J. M. Aung, Subhadeep Chowdhury, Shyama Prasad Saha et al.
SETTING: Greater Mymensingh District, a rural area of Bangladesh, at the start of the National Tuberculosis Programme (NTP). OBJECTIVES: To determine the prevalence of initial and acquired drug resistance of Mycobacterium tuberculosis, and to assess the appropriateness of the NTP's standard regimens...
Armand Van Deun, Tom Decroo, K. J. M. Aung, M. Anwar Hossain et al.
<b>Despite their name, borderline <i>rpoB</i> mutations are correlated with unfavourable outcomes when rifampicin-throughout treatment is used. They may become the drivers of rifampicin-resistant tuberculosis. Second-line treatment is recommended.</b> https://bit.ly/3nbkZjt
Zeaur Rahim, Khadiza Zaman, Adri G. M. van der Zanden, Marius J. Möllers et al.
ABSTRACT Spoligotyping was performed to study the population structure of Mycobacterium tuberculosis complex strains ( n = 224) from Bangladesh. Strains were split into principal genetic group 1 (PGG 1 [75.0%]) and PGG 2 and 3 (25%). Forty-nine strains with a new spoligotype signature and considered...
Armand Van Deun, AFM Salim, Paul Daru, Ananya Das et al.
SETTING: Greater Mymensingh District, Bangladesh. OBJECTIVES: To determine changes in prevalence of drug resistance of Mycobacterium tuberculosis under DOTS. DESIGN: Drug susceptibility testing of systematic samples of M. tuberculosis isolated from all sputum smear-positive cases newly registered in...
Isdore Chola Shamputa, Armand Van Deun, Md Abdul Hamid Salim, Md Anwar Hossain et al.
OBJECTIVE: To determine the relative frequencies of reinfection vs. reactivation or treatment failure in patients from a high tuberculosis incidence setting with a low prevalence of HIV infection. METHOD: We performed DNA fingerprinting on serial isolates from one and multiple TB episodes from 97 re...
Pauline Lempens, Tom Decroo, K. J. M. Aung, Mohammad Anwar Hossain et al.
OBJECTIVES: We investigated whether companion drug resistance was associated with adverse outcomes of the shorter multidrug-resistant tuberculosis (MDR-TB) treatment regimen in Bangladesh after adjustment for fluoroquinolone resistance. METHODS: MDR-TB/rifampicin-resistant tuberculosis patients regi...
K. J. M. Aung, Erik De Clercq, Md. A. Ali, Sowjanya Naha et al.
SETTING: Damien Foundation tuberculosis (TB) control projects in Bangladesh. OBJECTIVE: To assess the effectiveness of extending the intensive phase (P1) of treatment by 1 month for patients who are smear-positive after 2 months of a 6-month regimen containing rifampicin (RMP) throughout. DESIGN: Pr...