Junqiang Li, Zhenzhen Wang, Md Robiul Karim, Longxian Zhang
Diarrheal diseases caused by intestinal protozoan parasites are a major food-borne public health problem across the world. Vegetables and fruits provide important nutrients and minerals, but are also common sources of some food-borne human pathogenic microorganisms. The contamination of raw vegetabl...
Md Robiul Karim, Rongjun Wang, Haiju Dong, Longxian Zhang et al.
Enterocytozoon bieneusi is an important zoonotic pathogen. To assess the human-infective potential of E. bieneusi in nonhuman primates (NHPs), we examined the prevalence and genotype distribution of E. bieneusi in 23 NHP species by PCR and sequence analysis of the ribosomal internal transcribed spac...
Md Robiul Karim, Haiju Dong, Fuchang Yu, Fuchun Jian et al.
To explore the genetic diversity, host specificity, and zoonotic potential of Enterocytozoon bieneusi, feces from 348 stray and pet dogs and 96 pet cats from different locations in China were examined by internal transcribed spacer (ITS)-based PCR. E. bieneusi was detected in 15.5% of the dogs, incl...
Md Robiul Karim, Haiju Dong, Tongyi Li, Fuchang Yu et al.
To appreciate the genetic diversity and zoonotic implications of Enterocytozoon bieneusi in nonhuman primates (NHPs) in zoos, we genotyped E. bieneusi in captive NHPs in seven zoos located at six major cities in China, using ribosomal internal transcribed spacer (ITS)-based PCR and sequence analyses...
Md Robiul Karim, Sumei Zhang, Fuchun Jian, Jiacheng Li et al.
Non-human primates (NHPs) are commonly infected with Cryptosporidium spp. and Giardia duodenalis. However, molecular characterisation of these pathogens from NHPs remains scarce. In this study, 2,660 specimens from 26 NHP species in China were examined and characterised by PCR amplification of 18S r...
Md Robiul Karim, Fuchang Yu, Jian Li, Junqiang Li et al.
Enteric protozoa are frequently found in snakes. Nevertheless, few studies regarding genetic characterization of these parasites have been carried out. We describe here the first molecular survey of protozoan pathogens from snakes in China and the first report on Enterocytozoon bieneusi genotyping i...
Junqiang Li, Md Robiul Karim, Dongfang Li, SMMR Sumon et al.
Blastocystis sp. is a protozoan parasite, commonly found in the gastrointestinal tracts of animals and humans globally. The parasitic species has wide genetic diversity. Currently the mammalian and avian isolates of the parasite are grouped into 17 well known subtypes (STs), of which ten (ST1-ST9, S...
Md Robiul Karim, Rongjun Wang, Xiaoyi He, Longxian Zhang et al.
To infer population genetics of Enterocytozoon bieneusi in nonhuman primates (NHPs), 126 positive specimens in 839 fecal specimens from 23 NHP species in China based on ITS locus were used, belonging to genotypes Type IV, D, Peru8, Henan V, Peru11, PigEBITS7 and 3 novel ones (CM1, CM2 and CM3). Mult...
Md Robiul Karim, Rongjun Wang, Fuchang Yu, Tongyi Li et al.
Only a few studies based on single locus characterization have been conducted on the molecular epidemiology of Giardia duodenalis in nonhuman primates (NHPs). The present study was conducted to examine the occurrence and genotype identity of G. duodenalis in NHPs based on multi-locus analysis of the...
Teklu Wegayehu, Md Robiul Karim, Junqiang Li, Haileeyesus Adamu et al.
BACKGROUND: Cryptosporidium and Giardia duodenalis are gastro-intestinal parasites that infect human and animals worldwide. Both parasites share a broad host range and are believed to be zoonosis. The aim of this study was to identify the species of Cryptosporidium and assemblages of G. duodenalis i...
Dongfang Li, Shuangjian Zheng, Chunxiang Zhou, Md Robiul Karim et al.
Abstract Enterocytozoon bieneusi is one of the most frequently diagnosed Microsporidia of humans and most animals. However, there is no information on E. bieneusi infection of pigs in Tibet and Henan, China. In this study, 1,190 fecal samples were collected from pigs in Tibet and Henan and screened ...
Md Robiul Karim, Junqiang Li, Farzana Islam Rume, Sm. Mostafizur Rahaman Sumon et al.
Cryptosporidium and Giardia are protozoan parasites capable of causing gastrointestinal illness in humans and animals. The purpose of this research was to determine the occurrence, genetic characteristics, and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in captive mammals at th...
Md Robiul Karim, Farzana Islam Rume, Dongfang Li, Junqiang Li et al.
Enterocytozoon bieneusi is a widespread opportunistic pathogen found in humans and domestic animals, including cattle that poses a public health risk. This study was performed to evaluate the prevalence, genotypic diversity, and zoonotic potential of E. bieneusi among children and calves in Banglade...
Md Robiul Karim, Farzana Islam Rume, Md. Ataur Rahman, Zhenjie Zhang et al.
To determine the occurrence and genotypes of Enterocytozoon bieneusi in captive mammals at Bangladesh National Zoo and to assess their zoonotic significance, 200 fecal samples from 32 mammalian species were examined using a nested PCR and sequencing of internal transcribed spacer (ITS) gene. Enteroc...
Junqiang Li, Md Robiul Karim, Jun Li, Liping Zhang et al.
The giant panda (Ailuropoda melanoleuca) is a rare species with a small global population size, and lives in the wild in only a few fragmented mountain ranges of Southwest China. Parasitic infections are among the important causes of death of giant pandas that hamper their group development. We revi...