Rhea N. Coler, Malcolm S. Duthie, Kimberly A. Hofmeyer, Jeffery Guderian et al.
Key antigens of Leishmania species identified in the context of host responses in Leishmania-exposed individuals from disease-endemic areas were prioritized for the development of a subunit vaccine against visceral leishmaniasis (VL), the most deadly form of leishmaniasi Two Leishmania proteins-nu...
Faria Hossain, Prakash Ghosh, Md Anik Ashfaq Khan, Malcolm S. Duthie et al.
Sustained elimination of Visceral Leishmaniasis (VL) requires the reduction and control of parasite reservoirs to minimize the transmission of Leishmania donovani infection. A simple, reproducible and definitive diagnostic procedure is therefore indispensable for the early and accurate detection of ...
Aarthy C. Vallur, Yeung L. Tutterrow, Raodoh Mohamath, Sowmya Pattabhi et al.
BACKGROUND: Visceral leishmaniasis (VL) can be fatal without timely diagnosis and treatment. Treatment efficacies vary due to drug resistance, drug toxicity and co-morbidities. It is important to monitor treatment responsiveness to confirm cure and curtail relapse. Currently, microscopy of spleen, b...
Prakash Ghosh, Khondaker Rifat Hasan Bhaskar, Faria Hossain, Md Anik Ashfaq Khan et al.
BACKGROUND: Recombinant fusion proteins are now commonly used to detect circulating antibodies for the serodiagnosis of visceral leishmaniasis (VL) in Asia, Africa and the Americas. Although simple, these tests still require blood collection and their use in remote settings can be limited due to the...
Aarthy C. Vallur, Malcolm S. Duthie, C. Reinhart, Yeung L. Tutterrow et al.
Visceral leishmaniasis in South Asia is a serious disease affecting children and adults. Acute visceral leishmaniasis develops in only a fraction of those infected individuals, the majority being asymptomatic with the potential to transmit infection and develop disease. We followed 56 individuals ch...
Malcolm S. Duthie, Lais Da Silva Pereira, Michelle A. Favila, Kimberly A. Hofmeyer et al.
Abstract Vaccine development for vector-borne pathogens may be accelerated through the use of relevant challenge models, as has been the case for malaria. Because of the demonstrated biological importance of vector-derived molecules in establishing natural infections, incorporating natural challenge...
Aarthy C. Vallur, C. Reinhart, Raodoh Mohamath, Yasuyuki Goto et al.
Infection with Leishmania donovaniis typically asymptomatic, but a significant number of individuals may progress to visceral leishmaniasis (VL), a deadly disease that threatens 200 million people in areas where it is endemic. While diagnosis of acute VL has been simplified by the use of cost-effect...
Prakash Ghosh, Md Golam Hasnain, Faria Hossain, Md Anik Ashfaq Khan et al.
Abstract Background Post-kala-azar dermal leishmaniasis (PKDL) is a sequel to visceral leishmaniasis (VL), which is found in VL-endemic countries including Bangladesh. Because of these enigmatic cases, the success of the National Kala-azar Elimination Program is under threat. To date, diagnostic met...
Faria Hossain, Albert Picado, Sophie I. Owen, Prakash Ghosh et al.
With reduced prevalence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC), direct and field deployable diagnostic tests are needed to implement an effective diagnostic and surveillance algorithm for post-elimination VL control. In this regard, here we investigated the diagnostic effica...
Malcolm S. Duthie, Jeffery Guderian, Aarthy C. Vallur, Ajay Bhatia et al.
Until recently, chemotherapy for visceral leishmaniasis (VL; also known as kala-azar) was severely limited by factors such as high cost, route of administration, generation of side effects and potential for resistance. Although largely effective, chemotherapies have become available with the introdu...
Prakash Ghosh, Rajashree Chowdhury, Mohammad Enayet Hossain, Faria Hossain et al.
OBJECTIVES: The democratization of diagnostics is one of the key challenges towards containing the transmission of coronavirus disease 2019 (COVID-19) around the globe. The operational complexities of existing PCR-based methods, including sample transfer to advanced central laboratories with expensi...
Rajashree Chowdhury, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain et al.
To detect Post-kala-azar leishmaniasis (PKDL) cases, several molecular methods with promising diagnostic efficacy have been developed that involve complicated and expensive DNA extraction methods, thus limiting their application in resource-poor settings. As an alternative, we evaluated two rapid DN...
Dinesh Mondal, Prakash Ghosh, Rajashree Chowdhury, Christine Halleux et al.
INTRODUCTION: To sustain the achievement of kala-azar elimination program (KEP), early detection and treatment of the visceral leishmaniasis (VL) cases and associated modalities such as treatment failure (TF), relapse VL (RVL), and Post-kala-azar dermal leishmaniasis (PKDL) is the cornerstone. A pre...
Aarthy C. Vallur, A. Hailu, Dinesh Mondal, C. Reinhart et al.
Acute visceral leishmaniasis (VL) is caused by infection with parasites of the Leishmania donovani complex and may be fatal if not treated. Early diagnosis and efficacious treatment are the keys to effective VL management and control. Novel regimens are being developed to overcome limitations in VL ...
Prakash Ghosh, Rajashree Chowdhury, Shomik Maruf, Albert Picado et al.
Despite the availability of highly sensitive polymerase chain reaction (PCR)-based methods, the dearth of remotely deployable diagnostic tools circumvents the early and accurate detection of individuals with post-kala-azar dermal leishmaniasis (PKDL). Here, we evaluate a design-locked loop-mediated ...