Masatomo Morita, Makoto Ohnishi, Eiji Arakawa, N. A. Bhuiyan et al.
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
Munirul Alam, Nur A. Hasan, Marzia Sultana, G. Balakrish Nair et al.
The treatment regimen for diarrhea depends greatly on correct diagnosis of its etiology. Recent diarrhea outbreaks in Bangladesh showed Vibrio cholerae to be the predominant cause, although more than 40% of the suspected cases failed to show cholera etiology by conventional culture methods (CMs). In...
Md Mamun Monir, Mohammad Tarequl Islam, Razib Mazumder, Dinesh Mondal et al.
Abstract In 2022, one of its worst cholera outbreaks began in Bangladesh and the icddr,b Dhaka hospital treated more than 1300 patients and ca. 42,000 diarrheal cases from March-1 to April-10, 2022 1 . Here, we present genomic attributes of V. cholerae O1 responsible for the 2022 Dhaka outbreak and ...
Munirul Alam, Suraia Nusrin, Atiqul Islam, Nurul A. Bhuiyan et al.
Vibrio cholerae O1 biotype El Tor (ET), the cause of the current 7th pandemic, has recently been replaced in Asia and Africa by an altered ET biotype possessing cholera toxin (CTX) of the classical (CL) biotype that originally caused the first six pandemics before becoming extinct in the 1980s. Unti...
Daichi Morita, Masatomo Morita, Munirul Alam, Asish K. Mukhopadhyay et al.
Cholera continues to be a global concern, as large epidemics have occurred recently in Haiti, Yemen, and countries of sub-Saharan Africa. A single lineage of Vibrio cholerae O1 has been considered to be introduced into these regions from South Asia and to cause the spread of cholera. Using genomic e...
Nurul A. Bhuiyan, Suraia Nusrin, Munirul Alam, Masatomo Morita et al.
We determined the genotype of cholera toxin by amplifying and sequencing the B-subunit in a sequential collection of 90 strains of Vibrio cholerae O139 isolated over the past 13 years since its first description in 1992. Representative strains isolated during 1993-1997 harboured ctxB of El Tor type ...
Masatomo Morita, Shouji Yamamoto, Hirotaka Hiyoshi, Toshio Kodama et al.
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS-related genes were distributed among the various serogroups and pulsed-field gel electrophoresis of NotI-digested genomes showed genetic diversity in...
Md Mamun Monir, Talal Hossain, Masatomo Morita, Makoto Ohnishi et al.
Cholera is a global disease with specific reference to the Bay of Bengal Ganges Delta where Vibrio cholerae O1 El Tor, the causative agent of the disease showed two circulating lineages, one dominant in Bangladesh and the other in India. Results of an in-depth genomic study of V. cholerae associated...
Taichiro Takemura, Kazunori Murase, Fumito Maruyama, Thi Luong Tran et al.
Cholera epidemics have been recorded periodically in Vietnam during the seventh cholera pandemic. Since cholera is a water-borne disease, systematic monitoring of environmental waters for Vibrio cholerae presence is important for predicting and preventing cholera epidemics. We conducted monitoring, ...
Tania Tabassum Nisa, Yo Sugawara, Shigeto Hamaguchi, Dan Takeuchi et al.
Abstract Background The transmission of carbapenemase-producing Enterobacterales (CPE) in the external environment, especially through food, presents a significant public health risk. Objectives To investigate the prevalence and genetic characteristics of CPE in food markets of Dhaka, Bangladesh, us...
Danielle J. Ingle, Jane Hawkey, Martin Hunt, Zamin Iqbal et al.
Background: serovar Typhi (Typhi) and causes 10 million illnesses annually. Disease control relies on prevention (water, sanitation, and hygiene interventions or vaccination) and effective antimicrobial treatment. Antimicrobial resistant (AMR) Typhi lineages have emerged and become established in ma...
Danielle J. Ingle, Jane Hawkey, Martin Hunt, Zamin Iqbal et al.
BACKGROUND: Typhoid fever results from systemic infection with Salmonella enterica serovar Typhi (Typhi) and causes 10 million illnesses annually. Disease control relies on prevention (water, sanitation, and hygiene interventions or vaccination) and effective antimicrobial treatment. Antimicrobial-r...
Md Mamun Monir, Talal Hossain, Masatomo Morita, Makoto Ohnishi et al.
Abstract Comparative genomic analysis of Vibrio cholerae El Tor associated with endemic cholera in Asia revealed two distinct lineages, one dominant in Bangladesh and the other in India. An in depth whole genome study of V. cholerae El Tor clinical strains isolated during endemic cholera in Banglade...
Mohammad Tarequl Islam, Jarin Tasnim, Rabeya Basri, Mohammad Nazmus Sakib et al.
ABSTRACT The Ganges delta of the Bay of Bengal is a recognized hotspot for the emergence and spread of novel variants of Vibrio cholerae . Despite being a diverse species, very little information is available concerning environmental and human-associated aspects of V. cholerae serogroups, other than...
Ingle, Danielle J., Hawkey, Jane, Hunt, Martin, Iqbal, Zamin et al.
Additional file 1: Includes Fig S1 and S2 and Tables S7 and S8 (pdf). Table S1. Tabulated Typhi Mykrobe output table for all genomes included in validation analyses (available in GitHub). Table S2: Genome data used for validation (available in GitHub). Table S3. Details of AMR genotype calls compari...
Ingle, Danielle J., Hawkey, Jane, Hunt, Martin, Iqbal, Zamin et al.
Abstract Background Typhoid fever results from systemic infection with Salmonella enterica serovar Typhi (Typhi) and causes 10 million illnesses annually. Disease control relies on prevention (water, sanitation, and hygiene interventions or vaccination) and effective antimicrobial treatment. Antimic...
Ingle, Danielle J., Hawkey, Jane, Hunt, Martin, Iqbal, Zamin et al.
Additional file 1: Includes Fig S1 and S2 and Tables S7 and S8 (pdf). Table S1. Tabulated Typhi Mykrobe output table for all genomes included in validation analyses (available in GitHub). Table S2: Genome data used for validation (available in GitHub). Table S3. Details of AMR genotype calls compari...
Ingle, Danielle J., Hawkey, Jane, Hunt, Martin, Iqbal, Zamin et al.
Abstract Background Typhoid fever results from systemic infection with Salmonella enterica serovar Typhi (Typhi) and causes 10 million illnesses annually. Disease control relies on prevention (water, sanitation, and hygiene interventions or vaccination) and effective antimicrobial treatment. Antimic...
Munirul Alam, Md Mamun Monir, Mohammad Tarequl Islam, Razib Mazumder et al.
Abstract Bangladesh is currently experiencing one of the worst cholera outbreaks in its history. The icddr,b hospital has treated a record number of patients, more than 1400 per day and ca. 40,000 diarrheal cases from the end of March through April 20221. A recent genomic study showed temporal progr...
Masatomo Morita, Shouji Yamamoto, Hirotaka Hiyoshi, Toshio Kodama et al.
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS-related genes were distributed among the various serogroups and pulsed-field gel electrophoresis of NotI-digested genomes showed genetic diversity in...