Mohammadali Khan Mirzaei, Md Anik Ashfaq Khan, Prakash Ghosh, Zofia E. Taranu et al.
Stunting, a severe and multigenerational growth impairment, globally affects 22% of children under the age of 5 years. Stunted children have altered gut bacterial communities with higher proportions of Proteobacteria, a phylum with several known human pathogens. Despite the links between an altered ...
Dinesh Mondal, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain et al.
BACKGROUND: Leishmania donovani (LD) is a protozoan parasite transmitted to humans from sand flies, which causes Visceral Leishmaniasis (VL). Currently, the diagnosis is based on presence of the anti-LD antibodies and clinical symptoms. Molecular diagnosis would require real-time PCR, which is not e...
Faria Hossain, Prakash Ghosh, Md Anik Ashfaq Khan, Malcolm S. Duthie et al.
Sustained elimination of Visceral Leishmaniasis (VL) requires the reduction and control of parasite reservoirs to minimize the transmission of Leishmania donovani infection. A simple, reproducible and definitive diagnostic procedure is therefore indispensable for the early and accurate detection of ...
Prakash Ghosh, Khondaker Rifat Hasan Bhaskar, Faria Hossain, Md Anik Ashfaq Khan et al.
BACKGROUND: Recombinant fusion proteins are now commonly used to detect circulating antibodies for the serodiagnosis of visceral leishmaniasis (VL) in Asia, Africa and the Americas. Although simple, these tests still require blood collection and their use in remote settings can be limited due to the...
Md Anik Ashfaq Khan, Jenifar Quaiyum Ami, Khaledul Faisal, Rajashree Chowdhury et al.
BACKGROUND: Visceral leishmaniasis (VL) caused by dimorphic Leishmania species is a parasitic disease with high socioeconomic burden in endemic areas worldwide. Sustaining control of VL in terms of proper and prevailing immunity development is a global necessity amid unavailability of a prophylactic...
Malcolm S. Duthie, Lais Da Silva Pereira, Michelle A. Favila, Kimberly A. Hofmeyer et al.
Abstract Vaccine development for vector-borne pathogens may be accelerated through the use of relevant challenge models, as has been the case for malaria. Because of the demonstrated biological importance of vector-derived molecules in establishing natural infections, incorporating natural challenge...
Prakash Ghosh, Md Golam Hasnain, Faria Hossain, Md Anik Ashfaq Khan et al.
Abstract Background Post-kala-azar dermal leishmaniasis (PKDL) is a sequel to visceral leishmaniasis (VL), which is found in VL-endemic countries including Bangladesh. Because of these enigmatic cases, the success of the National Kala-azar Elimination Program is under threat. To date, diagnostic met...
Faria Hossain, Albert Picado, Sophie I. Owen, Prakash Ghosh et al.
With reduced prevalence of visceral leishmaniasis (VL) in the Indian subcontinent (ISC), direct and field deployable diagnostic tests are needed to implement an effective diagnostic and surveillance algorithm for post-elimination VL control. In this regard, here we investigated the diagnostic effica...
Mohammad Habibur Rahman, Salauddin Al Azad, Mohammad Fahim Uddin, Maisha Farzana et al.
The research aimed to establish a multidrug-resistant Klebsiella pneumoniae-induced genetic model for mastitis considering the alternative mechanisms of the DjlA-mediated CbpA protein regulation. The Whole Genome Sequencing of the newly isolated K. pneumoniae strain was conducted to annotate the fre...
Prakash Ghosh, Abhijit Sharma, Narayan Raj Bhattarai, Kumar Abhishek et al.
With the advancement of isothermal nucleic acid amplification techniques, detection of the pathogenic DNA in clinical samples at point-of-need is no longer a dream. The newly developed recombinase polymerase amplification (RPA) assay incorporated in a suitcase laboratory has shown promising diagnost...
Rajashree Chowdhury, Prakash Ghosh, Md Anik Ashfaq Khan, Faria Hossain et al.
To detect Post-kala-azar leishmaniasis (PKDL) cases, several molecular methods with promising diagnostic efficacy have been developed that involve complicated and expensive DNA extraction methods, thus limiting their application in resource-poor settings. As an alternative, we evaluated two rapid DN...
Benjawan Saechue, Naganori Kamiyama, Yinan Wang, Chiaki Fukuda et al.
Chikungunya fever is a mosquito-borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and simpl...
Dinesh Mondal, Prakash Ghosh, Rajashree Chowdhury, Christine Halleux et al.
INTRODUCTION: To sustain the achievement of kala-azar elimination program (KEP), early detection and treatment of the visceral leishmaniasis (VL) cases and associated modalities such as treatment failure (TF), relapse VL (RVL), and Post-kala-azar dermal leishmaniasis (PKDL) is the cornerstone. A pre...
Ashfaqul Hoque Khadem, Habibur Rahman Anik, Md Golam Mortuza Limon, Mahbubay Rabbani et al.
The necessity for achieving high-performance and precise detection of internal and external stimuli in physical and biological settings has emerged as exceedingly crucial in recent years. The exceptional electrical conductivity and high surface area of MXenes have significantly accelerated the advan...
Md Anik Ashfaq Khan, Prakash Ghosh, Rajashree Chowdhury, Faria Hossain et al.
The need for fast detection of etiological agents outside the narrow target range of pathogens that may cause an event of an infectious disease epidemic necessitates rapid sequencing technologies to be implemented in routine diagnostic procedures. We tested the performance of a PCR-free rapid nanopo...