Shah M. Faruque, Md Asadulghani, Manujendra N. Saha, A. R. M. A. Alim et al.
Toxigenic Vibrio cholerae strains are lysogens of CTXPhi, a filamentous phage which encodes cholera toxin. The receptor for CTXPhi for invading V. cholerae cells is the toxin-coregulated pilus (TCP), the genes for which reside in a larger genetic element, the TCP pathogenicity island. We analyzed 14...
Shah M. Faruque, Md Asadulghani, A. R. M. A. Alim, M. John Albert et al.
In toxigenic Vibrio cholerae, the CTX genetic element which carries the genes for cholera toxin (CT) is the genome of a lysogenic bacteriophage (CTXPhi). Clinical and environmental strains of V. cholerae O1 or O139 and stools that were culture positive for cholera were analyzed to study the inductio...
Shah M. Faruque, M. Mostafizur Rahman, Md Asadulghani, Kamrul Islam et al.
ABSTRACT The filamentous bacteriophage CTXΦ, which encodes cholera toxin (CT) in toxigenic Vibrio cholerae , is known to propagate by infecting susceptible strains of V. cholerae by using the toxin coregulated pilus (TCP) as its receptor and thereby causing the origination of new strains of toxigeni...
Shah M. Faruque, Md Asadulghani, Muhammad Kamruzzaman, Ranjan K. Nandi et al.
In toxigenic Vibrio cholerae, cholera toxin is encoded by the CTX prophage, which consists of a core region carrying ctxAB genes and genes required for CTXPhi morphogenesis, and an RS2 region encoding regulation, replication, and integration functions. Integrated CTXPhi is often flanked by another g...
Shah M. Faruque, Md Asadulghani, Mahmudur Rahman, Matthew K. Waldor et al.
In toxigenic Vibrio cholerae, the cholera enterotoxin (CT) is encoded by CTXPhi, a lysogenic bacteriophage. The propagation of this filamentous phage can result in the origination of new toxigenic strains. To understand the nature of possible environmental factors associated with the propagation of ...
Shah M. Faruque, Jun Zhu, Md Asadulghani, Muhammad Kamruzzaman et al.
The major virulence factors of toxigenic Vibrio cholerae are cholera toxin, which is encoded by a lysogenic filamentous bacteriophage (CTXPhi), and toxin-coregulated pilus (TCP), an essential colonization factor that is also the receptor for CTXPhi. The genes involved in the biosynthesis of TCP resi...
Shah M. Faruque, Abdullah Siddique, Manujendra N. Saha, Md Asadulghani et al.
Vibrio cholerae O139 Bengal initially appeared in the southern coastal region of Bangladesh and spread northward, causing explosive epidemics during 1992 and 1993. The resurgence of V. cholerae O139 during 1995 after its transient displacement by a new clone of El Tor vibrios demonstrated rapid chan...
Shah M. Faruque, Manujendra N. Saha, Md Asadulghani, Prasanta K. Bag et al.
In order to assess the extent of genomic diversity among Vibrio cholerae O139 strains, restriction fragment length polymorphisms in two genetic loci, rrn and ctx, were studied. Analysis of 144 strains isolated from different regions of Bangladesh and India between 1992 and 1998 revealed the presence...
Shah M. Faruque, Manujendra N. Saha, Md Asadulghani, David A. Sack et al.
Sixty-four representative strains of Vibrio cholerae O139 were analyzed, to re-examine the origin of this serogroup. Ribotyping differentiated the strains into 3 HindIII and 7 BglI ribotypes. One HindIII and 5 BglI ribotypes were shared by all toxigenic O139 strains. Of 6 nontoxigenic O139 strains, ...
Shah M. Faruque, Muhammad Kamruzzaman, Md Asadulghani, David A. Sack et al.
The cholera toxin genes of Vibrio cholerae are encoded by the filamentous phage, CTXphi. Chromosomal CTXphi prophage DNA is often found flanked by copies of a related genetic element designated RS1, and RS1 DNA can be packaged into filamentous phage particles (designated RS1phi) by using the CTXphi ...
Arif Hussain, Razib Mazumder, Abdullah Ahmed, Umme Saima et al.
Klebsiella pneumoniae is recognized as an urgent public health threat because of the emergence of difficult-to-treat (DTR) strains and hypervirulent clones, resulting in infections with high morbidity and mortality rates. Despite its prominence, little is known about the genomic epidemiology of K. p...
Mahdia Rahman, Ashikun Nabi, Md Asadulghani, Shah M. Faruque et al.
In many Asian countries including Bangladesh E. coli O157 are prevalent in animal reservoirs and in the food chain, but the incidence of human infection due to E. coli O157 is rare. One of the reasons could be inability of the organism from animal origin to produce sufficient amount of Shiga toxin (...
Razib Mazumder, Arif Hussain, Jody Phelan, Susana Campino et al.
Multi-resistant pathogenic strains of non-lactose fermenting Escherichia coli (NLF E. coli ) are responsible for various intestinal and extraintestinal infections. Although several studies have characterised such strains using conventional methods, they have not been comprehensively studied at the g...
Shakhinur Islam Mondal, Md Rakibul Islam, Akira Sawaguchi, Md Asadulghani et al.
Shiga toxin 2 (Stx2), one of the most important virulence factors of enterohaemorrhagic Escherichia coli (EHEC), is encoded by phages. These phages (Stx2 phages) are often called lambda-like. However, most Stx2 phages are short-tailed, thus belonging to the family Podoviridae, and the functions of m...
Arif Hussain, Razib Mazumder, Md Asadulghani, Taane G. Clark et al.
Md Asadulghani, Pawan Angra, Md Giasuddin, Md. Latiful Bari et al.
Introduction: Many emerging and reemerging pathogens have been identified as major public health threats in Bangladesh. Collection, transportation, and storage of infectious materials and management of generated waste from diagnosing those diseases require strict adherence to biosafety and biosecuri...
Md Asadulghani, Natasha K. Griffith, William Arndt
• Before introduction of antimicrobials, in HICs, various public health interventions, surveillance and infectious diseases control measures, were mostly responsible for the largest improvements in life expectancy. • Antimicrobials alone are being used in many LMICs to reduce the morbidity and morta...
Suruchi Aggarwal, Faizan Ahmed, Rajani Chowdary Akkina, Gunjan Arora et al.