Elizabeth A. Ashley, Mehul Dhorda, Rick M. Fairhurst, Chanaki Amaratunga et al.
BACKGROUND: Artemisinin resistance in Plasmodium falciparum has emerged in Southeast Asia and now poses a threat to the control and elimination of malaria. Mapping the geographic extent of resistance is essential for planning containment and elimination strategies. METHODS: Between May 2011 and Apri...
Olivo Miotto, Roberto Amato, Elizabeth A. Ashley, Bronwyn MacInnis et al.
We report a large multicenter genome-wide association study of Plasmodium falciparum resistance to artemisinin, the frontline antimalarial drug. Across 15 locations in Southeast Asia, we identified at least 20 mutations in kelch13 (PF3D7_1343700) affecting the encoded propeller and BTB/POZ domains, ...
Sachel Mok, Elizabeth A. Ashley, Pedro Eduardo Ferreira, Lei Zhu et al.
Artemisinin resistance in Plasmodium falciparum threatens global efforts to control and eliminate malaria. Polymorphisms in the kelch domain-carrying protein K13 are associated with artemisinin resistance, but the underlying molecular mechanisms are unknown. We analyzed the in vivo transcriptomes of...
Kyaw Myo Tun, Mallika Imwong, Khin Maung Lwin, Aye Aye Win et al.
BACKGROUND: Emergence of artemisinin resistance in southeast Asia poses a serious threat to the global control of Plasmodium falciparum malaria. Discovery of the K13 marker has transformed approaches to the monitoring of artemisinin resistance, allowing introduction of molecular surveillance in remo...
Rob W. van der Pluijm, Rupam Tripura, Richard M. Hoglund, Aung Pyae Phyo et al.
BACKGROUND: Artemisinin and partner-drug resistance in Plasmodium falciparum are major threats to malaria control and elimination. Triple artemisinin-based combination therapies (TACTs), which combine existing co-formulated ACTs with a second partner drug that is slowly eliminated, might provide eff...
Hsiao‐Han Chang, Amy Wesolowski, Ipsita Sinha, Christopher G. Jacob et al.
For countries aiming for malaria elimination, travel of infected individuals between endemic areas undermines local interventions. Quantifying parasite importation has therefore become a priority for national control programs. We analyzed epidemiological surveillance data, travel surveys, parasite g...
Christopher G. Jacob, Thuy-Nhien Nguyen, Mayfong Mayxay, Richard J. Maude et al.
Background: National Malaria Control Programmes (NMCPs) currently make limited use of parasite genetic data. We have developed GenRe-Mekong, a platform for genetic surveillance of malaria in the Greater Mekong Subregion (GMS) that enables NMCPs to implement large-scale surveillance projects by integ...
Ricardo Ataíde, Elizabeth A. Ashley, Rosanna Powell, Jo-Anne Chan et al.
Significance Slow-clearing artemisinin-resistant malaria parasites are now well established in the Greater Mekong Subregion. This large multinational therapy efficacy study incorporating clinical data, molecular drug-resistance markers, and immune profiling aimed to understand how variations in popu...
Amol C. Shetty, Christopher G. Jacob, Fang Huang, Yao Li et al.
Estimates of Plasmodium falciparum migration may inform strategies for malaria elimination. Here we elucidate fine-scale parasite population structure and infer recent migration across Southeast Asia using identity-by-descent (IBD) approaches based on genome-wide single nucleotide polymorphisms call...
Lei Zhu, Jaishree Tripathi, Frances Rocamora, Olivo Miotto et al.
The predisposition of parasites acquiring artemisinin resistance still remains unclear beyond the mutations in Pfk13 gene and modulation of the unfolded protein response pathway. To explore the chain of casualty underlying artemisinin resistance, we reanalyze 773 P. falciparum isolates from TRACI-st...
Lei Zhu, Rob W. van der Pluijm, Michal Kucharski, Sourav Nayak et al.
The emergence and spread of artemisinin-resistant Plasmodium falciparum, first in the Greater Mekong Subregion (GMS), and now in East Africa, is a major threat to global malaria elimination ambitions. To investigate the artemisinin resistance mechanism, transcriptome analysis was conducted of 577 P....
Debashish Das, Ranitha Vongpromek, Thanawat Assawariyathipat, Ketsanee Srinamon et al.
BACKGROUND: Microscopic examination of Giemsa-stained blood films remains the reference standard for malaria parasite detection and quantification, but is undermined by difficulties in ensuring high-quality manual reading and inter-reader reliability. Automated parasite detection and quantification ...
Eric P.M. Grist, Jennifer A. Flegg, Georgina Humphreys, Ignacio Más et al.
BACKGROUND: Artemisinin-resistant Plasmodium falciparum malaria parasites are now present across much of mainland Southeast Asia, where ongoing surveys are measuring and mapping their spatial distribution. These efforts require substantial resources. Here we propose a generic 'smart surveillance' me...
Christopher G. Jacob, Thuy-Nhien Nguyen, Mayfong Mayxay, Richard J. Maude et al.
ABSTRACT National Malaria Control Programmes (NMCPs) currently make limited use of parasite genetic data. We have developed GenRe-Mekong, a platform for genetic surveillance of malaria in the Greater Mekong Subregion (GMS) that enables NMCPs to implement large-scale surveillance projects by integrat...
Katherine O’Flaherty, Jo-Anne Chan, Julia C. Cutts, Sophie Zaloumis et al.
Introduction Understanding the human immune response to Plasmodium falciparum gametocytes and its association with gametocytemia is essential for understanding the transmission of malaria as well as progressing transmission blocking vaccine candidates. Methods In a multi-national clinical efficacy t...