Hasan Mehraj, Ayasha Akter, Naomi Miyaji, Junji Miyazaki et al.
The genus Brassica contains important vegetable crops, which serve as a source of oil seed, condiments, and forages. However, their production is hampered by various diseases such as clubroot and Fusarium wilt, especially in Brassica vegetables. Soil-borne diseases are difficult to manage by traditi...
Hasan Mehraj, Satoshi Takahashi, Naomi Miyaji, Ayasha Akter et al.
Covalent modifications of histone proteins act as epigenetic regulators of gene expression. We report the distribution of two active histone marks (H3K4me3 and H3K36me3) in 14-day leaves in two lines of Brassica rapa L. by chromatin immunoprecipitation sequencing. Both lines were enriched with H3K4m...
Ayasha Akter, Junji Miyazaki, Daniel J. Shea, Namiko Nishida et al.
Chinese cabbage (Brassica rapa L. var. pekinensis) is an economically and agriculturally significant leafy vegetable that is extensively cultivated throughout the world. Vernalization is exposure to prolonged cold that alters gene expression and accelerates a transition from the vegetative to reprod...
Mst. Arjina Akter, Hasan Mehraj, Naomi Miyaji, Satoshi Takahashi et al.
Long noncoding RNAs (lncRNAs) play important roles in abiotic and biotic stress responses; however, studies on the mechanism of regulation of lncRNA expression are limited in plants. The present study examined the relationship between lncRNA expression level and two active histone modifications (H3K...
Maho Sato, Motoki Shimizu, Daniel J. Shea, Mozammel Hoque et al.
The fusarium yellows resistance (YR) gene FocBo1 was previously identified and the DNA markers were developed to assist the breeding of YR cultivars in Brassica oleracea. However, the further analysis revealed discrepancies between the phenotypes and the genotypes predicted by those DNA markers in c...
Soodeh Tirnaz, Naomi Miyaji, Shohei Takuno, Philipp E. Bayer et al.
DNA methylation is an epigenetic mark associated with several mechanisms in plants including immunity mechanisms. However, little is known about the regulatory role of DNA methylation in the resistance response of Brassica species against fungal diseases. White rust, caused by the fungus Albugo cand...
Hasan Mehraj, Daniel J. Shea, Satoshi Takahashi, Naomi Miyaji et al.
Long noncoding RNAs (lncRNAs) are RNA fragments that generally do not code for a protein but are involved in epigenetic gene regulation. In this study, lncRNAs of Brassica rapa were classified into long intergenic noncoding RNAs, natural antisense RNAs, and intronic noncoding RNAs and their expressi...
T. Okamoto, Xiaochun Wei, Hasan Mehraj, Mohammad Rashed Hossain et al.
Naomi Miyaji, Mst. Arjina Akter, Motoki Shimizu, Hasan Mehraj et al.
Albugo candida causing white rust disease decreases the yield of Brassica rapa vegetables greatly. Resistant and susceptible cultivars in B. rapa vegetables have different immune responses against A. candida inoculation, however, the mechanism of how host plants respond to A. candida is still unknow...
Naomi Miyaji, Mst. Arjina Akter, Chizuko Suzukamo, Hasan Mehraj et al.
In vegetables of Brassica rapa L., Fusarium oxysporum f. sp. rapae (For) or F. oxysporum f. sp. conglutinans (Foc) cause Fusarium yellows. A resistance gene against Foc (FocBr1) has been identified, and deletion of this gene results in susceptibility (focbr1-1). In contrast, a resistance gene agains...
Hasan Mehraj, Takahiro Kawanabe, Motoki Shimizu, Naomi Miyaji et al.
Heterosis or hybrid vigour is a phenomenon in which hybrid progeny exhibit superior yield and biomass to parental lines and has been used to breed F1 hybrid cultivars in many crops. A similar level of heterosis in all F1 individuals is expected as they are genetically identical. However, we found va...
Renmin Wu, Mei Iwamura, Naomi Miyaji, Satoshi Takahashi et al.
DNA methylation is a key epigenetic mechanism that regulates gene expression. The level and distribution of DNA methylation have been suggested to contribute to disease resistance and may contribute to differences in disease resistance between cultivars. In this study, we investigated whether inter-...
Mei Iwamura, Renmin Wu, Naomi Miyaji, Ayasha Akter et al.
DNA methylation is a key epigenetic modification essential for gene regulation, transposable element (TE) silencing, and genome stability. Genome-wide methylation profiling is now widely used in plant research, with whole-genome bisulfite sequencing (WGBS) being the most established method. However,...
Hasan Mehraj, Takahiro Kawanabe, Motoki Shimizu, Naomi Miyaji et al.
Heterosis or hybrid vigour is a phenomenon in which hybrid progeny exhibit superior yield and biomass to parental lines and has been used to breed F1 hybrid cultivars in many crops. A similar level of heterosis in all F1 individuals is expected as they are genetically identical. However, we found va...