A. Huq, E. B. Small, P. A. West, Mohsina Huq et al.
Strains of Vibrio cholerae, both O1 and non-O1 serovars, were found to attach to the surfaces of live copepods maintained in natural water samples collected from the Chesapeake Bay and Bangladesh environs. The specificity of attachment of V. cholerae to live copepods was confirmed by scanning electr...
Mercedes Pascual, Xavier Rodó, Stephen P. Ellner, Rita R. Colwell et al.
Analysis of a monthly 18-year cholera time series from Bangladesh shows that the temporal variability of cholera exhibits an interannual component at the dominant frequency of El Niño-Southern Oscillation (ENSO). Results from nonlinear time series analysis support a role for both ENSO and previous d...
B. Lobitz, Louisa R. Beck, Anwar Huq, Byron L. Wood et al.
It has long been known that cholera outbreaks can be initiated when Vibrio cholerae, the bacterium that causes cholera, is present in drinking water in sufficient numbers to constitute an infective dose, if ingested by humans. Outbreaks associated with drinking or bathing in unpurified river or brac...
Rita R. Colwell, Anwar Huq, M. Sirajul Islam, K. M. A. Aziz et al.
Based on results of ecological studies demonstrating that Vibrio cholerae, the etiological agent of epidemic cholera, is commensal to zooplankton, notably copepods, a simple filtration procedure was developed whereby zooplankton, most phytoplankton, and particulates >20 microm were removed from wate...
Jongsik Chun, Christopher J. Grim, Nur A. Hasan, Jehee Lee et al.
Vibrio cholerae, the causative agent of cholera, is a bacterium autochthonous to the aquatic environment, and a serious public health threat. V. cholerae serogroup O1 is responsible for the previous two cholera pandemics, in which classical and El Tor biotypes were dominant in the sixth and the curr...
Mark L. Tamplin, Anne L. Gauzens, Anwar Huq, David A. Sack et al.
Vibrio cholerae serogroup O1, the causative agent of cholera, is capable of surviving in aquatic environments for extended periods and is considered an autochthonous species in estuarine and brackish waters. These environments contain numerous elements that may affect its ecology. The studies report...
Guillaume Constantin de Magny, Raghu Murtugudde, Mathew R. P. Sapiano, Azhar Nizam et al.
The causative agent of cholera, Vibrio cholerae , has been shown to be autochthonous to riverine, estuarine, and coastal waters along with its host, the copepod, a significant member of the zooplankton community. Temperature, salinity, rainfall and plankton have proven to be important factors in the...
Anwar Huq, R. Bradley Sack, Azhar Nizam, Ira M. Longini et al.
The occurrence of outbreaks of cholera in Africa in 1970 and in Latin America in 1991, mainly in coastal communities, and the appearance of the new serotype Vibrio cholerae O139 in India and subsequently in Bangladesh have stimulated efforts to understand environmental factors influencing the growth...
A. Huq, Rita R. Colwell, R Rahman, Afsar Ali et al.
Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites ...
Munirul Alam, Marzia Sultana, G. Balakrish Nair, Abul K.M. Siddique et al.
Vibrio cholerae persists in aquatic environments predominantly in a nonculturable state. In this study coccoid, nonculturable V. cholerae O1 in biofilms maintained for 495 days in Mathbaria, Bangladesh, pond water became culturable upon animal passage. Culturability, biofilm formation, and the wbe, ...
R. Bradley Sack, Abdullah Siddique, Ira M. Longini, Azhar Nizam et al.
How Vibrio cholerae spreads around the world and what determines its seasonal peaks in endemic areas are not known. These features of cholera have been hypothesized to be primarily the result of environmental factors associated with aquatic habitats that can now be identified. Since 1997, fortnightl...
Nur A. Hasan, Seon Young Choi, Mark Eppinger, Philip Wilson Clark et al.
The millions of deaths from cholera during the past 200 y, coupled with the morbidity and mortality of cholera in Haiti since October 2010, are grim reminders that Vibrio cholerae, the etiologic agent of cholera, remains a scourge. We report the isolation of both V. cholerae O1 and non-O1/O139 early...
Shah M. Faruque, David A. Sack, R. Bradley Sack, Rita R. Colwell et al.
The emergence of Vibrio cholerae O139 Bengal during 1992-1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V. cholerae O1 strains. However, the O1 strains reemerged in 1994 and initiated a series of disappearance and...
Soumen Chakraborty, Asish K. Mukhopadhyay, Rupak K. Bhadra, Amar N. Ghosh et al.
The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining virulence in Vibrio cholerae, and these genes are believed to be exclusively associated with clinical stra...
Munirul Alam, Marzia Sultana, G. Balakrish Nair, R. Bradley Sack et al.
Toxigenic Vibrio cholerae, rarely isolated from the aquatic environment between cholera epidemics, can be detected in what is now understood to be a dormant stage, i.e., viable but nonculturable when standard bacteriological methods are used. In the research reported here, biofilms have proved to be...