Abdullah H Baqui, Shams El Arifeen, Gary L. Darmstadt, Saifuddin Ahmed et al.
BACKGROUND Neonatal mortality accounts for a high proportion of deaths in children under the age of 5 years in Bangladesh. Therefore the project for advancing the health of newborns and mothers (Projahnmo) implemented a community-based intervention package through government and non-government organ...
Rashidul Haque, Shantanu Roy, Abdullah Siddique, Utpal K. Mondal et al.
Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. are not only three of the most important and common diarrhea-causing parasitic protozoa, but they often have similar clinical presentations. Microscopic diagnosis of these parasites is neither sensitive nor specific. Recently, mor...
Rashidul Haque, Mamun Kabir, Zannatun Noor, S. M. Mazidur Rahman et al.
The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evalu...
Arfatur Rahman, Mahfuza Sahrin, Sadia Afrin, Keith T. Earley et al.
BACKGROUND: GeneXpert MTB/RIF (Xpert) and Genotype MTBDRplus (DRplus) are two World Health Organization (WHO) endorsed probe based molecular drug susceptibility testing (DST) methods for rapid diagnosis of drug resistant tuberculosis. Both methods target the same 81 bp Rifampicin Resistance Determin...
Sayera Banu, S. M. Mazidur Rahman, Md. Siddiqur Rahman Khan, Sara Sabrina Ferdous et al.
Given the increases in drug-resistant tuberculosis, laboratory capacities for drug susceptibility testing are being scaled up worldwide. A laboratory must decide among several endorsed methodologies. We evaluated 87 Mycobacterium tuberculosis isolates for concordance of susceptibility results across...
Senjuti Kabir, S. M. Mazidur Rahman, Shakil Ahmed, Md Shamiul Islam et al.
BACKGROUND: The World Health Organization recommends the Xpert MTB/RIF Ultra assay for diagnosing pulmonary tuberculosis (PTB) in children. Though stool is a potential alternative to respiratory specimens among children, the diagnostic performance of Xpert Ultra on stool is unknown. Thus, we assesse...
Mohammad Khaja Mafij Uddin, Tahmina Shirin, Mohammad Enayet Hossain, Ahmed Nawsher Alam et al.
As the COVID-19 pandemic erupted, the WHO recommended the use of nasopharyngeal or throat swabs for the detection of SARS-CoV-2 etiology of COVID-19. The collection of NPS causes discomfort because of its invasive collection procedure. There are considerable risks to health care workers during the c...
S. M. Mazidur Rahman, Umme Tasnim Maliha, Shahriar Ahmed, Senjuti Kabir et al.
BACKGROUND: The Xpert MTB/RIF (Xpert) assay technology allows rapid and sensitive diagnosis of pulmonary tuberculosis (PTB) from sputum specimens. However, diagnosis of PTB is difficult for patients who cannot produce sputum. The objective of this study was to investigate the use of Xpert assay for ...
Suporn Pholwat, Jie Liu, Suzanne Stroup, Jean Gratz et al.
UNLABELLED: Genotypic methods for drug susceptibility testing of Mycobacterium tuberculosis are desirable to speed the diagnosis and proper therapy of tuberculosis (TB). However, the numbers of genes and polymorphisms implicated in resistance have proliferated, challenging diagnostic design. We deve...
Mohammad Khaja Mafij Uddin, Arfatur Rahman, Md. Fahim Ather, Tanvir Ahmed et al.
Background: Rifampicin resistance (RR) is a key indicator of multidrug-resistant tuberculosis (MDR-TB) and 95% of the RR is associated with the mutation in the 81-bp rifampicin resistance determining region (RRDR) of the rpoB gene of Mycobacterium tuberculosis complex (MTBC). The Xpert MTB/RIF (Xper...
Sayera Banu, Farhana Haque, Shahriar Ahmed, Sonia Sultana et al.
BACKGROUND: In Bangladesh, about 80% of healthcare is provided by the private sector. Although free diagnosis and care is offered in the public sector, only half of the estimated number of people with tuberculosis are diagnosed, treated, and notified to the national program. Private sector engagemen...
Scott K. Heysell, Shahriar Ahmed, Sara Sabrina Ferdous, Md. Siddiqur Rahman Khan et al.
BACKGROUND: Multidrug-resistant tuberculosis (MDR-TB) treatment in Bangladesh is empiric or based on qualitative drug-susceptibility testing (DST) by comparative growth in culture media with and without a single drug concentration. METHODS: Adult patients were enrolled throughout Bangladesh during t...
Arfatur Rahman, Sara Sabrina Ferdous, Shahriar Ahmed, S. M. Mazidur Rahman et al.
ABSTRACT Pyrazinamide (PZA) is a frontline antituberculosis (anti-TB) drug used in both first- and second-line treatment regimens. However, due to complex laboratory requirements, the PZA susceptibility test is rarely performed, leading to a scarcity of data on susceptibility to PZA. Bangladesh is a...
Suporn Foongladda, Sayera Banu, Suporn Pholwat, Jean Gratz et al.
BACKGROUND: Although phenotypic drug susceptibility testing (DST) is endorsed as the standard for second-line drug testing of Mycobacterium tuberculosis, it is slow and laborious. METHODS: We evaluated the accuracy of two faster, easier methodologies that provide results for multiple drugs: a genoty...
Claudia Sala, Andrej Benjak, Delia Goletti, Sayera Banu et al.
The impact of tuberculosis and of anti-tuberculosis therapy on composition and modification of human lung microbiota has been the object of several investigations. However, no clear outcome has been presented so far and the relationship between M. tuberculosis pulmonary infection and the resident lu...