P-1999. Development and Qualification of IgM and IgG ELISAs for Nipah Virus Detection and Analysis of Human Antibody Response Dynamics

Abstract Background Nipah virus (NiV) poses a major public health threat due to severe disease and diagnostic challenges. This study focuses on developing and qualifying enzyme-linked immunosorbent assays ELISAs for detecting NiV-specific IgG and IgM antibodies, supporting retrospective diagnosis, serosurveillance, and vaccine evaluation. We aimed to develop and qualify in-house indirect ELISA assays for detecting anti-Nipah IgG and IgM antibodies using recombinant glycoprotein G, and to assess the duration of IgM and IgG responses throughout infection and recovery in RT-PCR–confirmed human NiV cases.Title: Different concentrations of coating antigen and conjugate with stop solution at 15 minutes combinationsa) 0.5 ug/ml coating and 1:500 conjugate dilution b) 2.0 ug/ml coating and 1:500 conjugate dilution c) 3.0 ug/ml coating and 1:500 conjugate dilution…