Thymosin Alpha-1 Restores Chemotherapy-Induced Antitumor Immunity by Chaperoning a MicroRNA Ligand of TLR7 in Dendritic Cells

Chemotherapy induces cancer cell apoptosis and the release of apoptotic bodies (ABs) that are poorly immunogenic or immunosuppressive, creating a major barrier to the success of co-administered or second-line immunotherapies. Here, we found reduced circulating levels of thymosin alpha-1 (Tα-1), a key endogenous peptide hormone with immunomodulatory activity, after chemotherapy treatment in patients with multiple types of cancer and mice bearing established tumors. Tα-1 bound to tumor ABs and interacted with AB-borne microRNAs, including miR146a-5p, following phagocytosis of ABs into the endolysosomal compartment of dendritic cells (DCs). The interaction with Tα-1 protected miR146a-5p from lysosomal RNase A-mediated degradation, allowing miR146a-5p-mediated activation of Toll-like receptor 7 (TLR7) that licenses DC maturation, migration to tumor-draining lymph nodes, and presentation of tumor antigens…