MicroRNA-186-5p overexpression modulates colon cancer growth by repressing the expression of the FAM134B tumour inhibitor

Objectives: The role and underlying mechanism of miR‐186‐5p in colorectal cancer remain unknown. The present study aims to examine the various cellular effects of miR‐186‐5p in the carcinogenesis of colorectal cancer. Also, the interacting targets and association of clinicopathological factors with miR‐186‐5p expression in patients with colorectal cancer were analysed. Methods: The miR‐186‐5p expression levels in colorectal cancer tissues (n=126) and colon cancer cell lines (n=3) were analysed by real‐time PCR. Matched non‐neoplastic colorectal tissues and a non‐neoplastic colonic epithelial cell line were used as controls. Various in vitro assays such as cell proliferation, wound healing and colony formation assays were performed to examine the miR‐186‐5p specific cellular effects. Western blots and immunohistochemistry analysis were performed to examine the modulation…