Rashidul Haque, Abu Syed Golam Faruque, P Hahn, David M. Lyerly et al.
The prevalence of infection by the invasive parasite Entamoeba histolytica and the noninvasive parasite Entamoeba dispar was determined in 2000 children in Bangladesh. Antigen detection identified more cases of E. histolytica-E. dispar infection than did culture or microscopy. Microscopic identifica...
Shantanu Roy, Mamun Kabir, Dinesh Mondal, Ibne Karim M. Ali et al.
We developed a real-time-PCR assay utilizing a molecular-beacon probe for the detection of Entamoeba histolytica and compared its sensitivity to stool antigen detection and traditional PCR. A total of 205 stool and liver abscess pus specimens from patients and controls were used for this purpose, 10...
Rashidul Haque, Priya Duggal, Ibnekarim M. Ali, Mohammad Bakthiar Hossain et al.
Entamoeba histolytica infection and colitis occurred in 55% and 4%, respectively, of a cohort of Bangladeshi preschool children observed for 2 years. DNA typing demonstrated that infecting E. histolytica isolates were genetically diverse. Innate resistance to infection in children was linked to the ...
Junqiang Li, Zhenzhen Wang, Md Robiul Karim, Longxian Zhang
Diarrheal diseases caused by intestinal protozoan parasites are a major food-borne public health problem across the world. Vegetables and fruits provide important nutrients and minerals, but are also common sources of some food-borne human pathogenic microorganisms. The contamination of raw vegetabl...
Dinesh Mondal, Rashidul Haque, R. Bradley Sack, Beth D. Kirkpatrick et al.
We examined whether malnutrition (underweight [WAZ] < -2) increased the risk of diarrhea equally for all enteropathogens. The study was conducted prospectively between January 1999 and July 2002 in Mirpur, an urban slum in Dhaka. Two hundred eighty-nine Bangladeshi children (147 male and 142 female)...
W D B Clements, Rowan W. Parks, P Erwin, M I Halliday et al.
BACKGROUND: Gram negative septic events are the commonest source of morbidity and mortality as a result of surgery in jaundiced patients. The large intestine provides the major source of Gram negative bacteria in mammals and is implicated in the pathogenesis of systemic endotoxaemia in obstructive j...
Shurong Dong, Ya Yang, Yingjian Wang, Dongjian Yang et al.
BACKGROUND: Cryptosporidium is a protozoan parasite that can infect both humans and animals and cause cryptosporidiosis. We aimed to estimate the global prevalence of Cryptosporidium infection. METHODS: In this study, Web of Science, Medline and PubMed were searched for relative articles, published ...
Armando González-Ruiz, Rashidul Haque, Aura Aguirre, G Castañón et al.
AIMS: To assess the reliability of the detection of erythrophagocytic amoebic trophozoites in stool samples in the diagnosis of dysentery associated with invasive Entamoeba histolytica. METHODS: Amoebic culture was carried out on single stool samples collected from patients from Mexico, Colombia, an...
Roberta M. O’Connor, Rehaan Shaffie, Gagandeep Kang, Honorine Ward
Introduction Cryptosporidium spp. are intestinal protozoan parasites of the phylum Apicomplexa, which cause diarrheal disease in humans worldwide (reviewed in [1–6]). In immunocompetent individuals, infection with this parasite may be asymptomatic or cause a self-limiting diarrheal illness. However,...
Gretchen Ehrenkaufer, Rashidul Haque, Jason A. Hackney, Daniel Eichinger et al.
Developmental switching between life-cycle stages is a common feature among many pathogenic organisms. The protozoan parasite Entamoeba histolytica converts between cysts (essential for disease transmission) and trophozoites (responsible for tissue invasion). Identification of genes involved in the ...
Carol A. Gilchrist, Sarah E. Petri, Brittany N. Schneider, Daniel Reichman et al.
BACKGROUND: An estimated 1 million children die each year before their fifth birthday from diarrhea. Previous population-based surveys of pediatric diarrheal diseases have identified the protozoan parasite Entamoeba histolytica, the etiological agent of amebiasis, as one of the causes of moderate-to...
Md Robiul Karim, Haiju Dong, Fuchang Yu, Fuchun Jian et al.
To explore the genetic diversity, host specificity, and zoonotic potential of Enterocytozoon bieneusi, feces from 348 stray and pet dogs and 96 pet cats from different locations in China were examined by internal transcribed spacer (ITS)-based PCR. E. bieneusi was detected in 15.5% of the dogs, incl...
Dinesh Mondal, William A. Petri, R. Bradley Sack, Beth D. Kirkpatrick et al.
The enteric protozoa, Cryptosporidium, Giardia and Entamoeba histolytica, cause diarrhea in children. We investigated the association of enteric protozoan-associated diarrheal illness with the nutritional status and growth of preschool children in Dhaka, Bangladesh. The subjects were 221 children ag...
Rashidul Haque, K. Kress, Sheila Wood, Terry Jackson et al.
Monoclonal antibodies (MAbs) directed against pathogen-specific epitopes of the galactose adhesin of Entamoeba histolytica were used in an ELISA to detect antigen from pathogenic E. histolytica. Single stool specimens from 74 patients in Bangladesh were used. The ELISA for pathogenic E. histolytica ...