Rashidul Haque, Ibne Karim M. Ali, Selim Akther, William A. Petri
The diagnosis of amebiasis by microscopic identification of the parasite in stool is insensitive and unable to distinguish the invasive parasite Entamoeba histolytica from the commensal parasite E. dispar. In this study, we have tested a PCR technique for the detection of E. histolytica and compared...
Rashidul Haque, Nasir Uddin Mollah, Ibne Karim M. Ali, Khorshed Alam et al.
A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identify Entamoeba histolytica in stool specimens from patients with amebic liver abscess. Here we repor...
Motohiro Iseki, Ibne Karim M. Ali, Momammad B. Hossain, Viqar Zaman et al.
Since the genotype of human Blastocystis hominis isolates is highly polymorphic, PCR-based genotype classification using known sequenced-tagged site (STS) primers would allow the identification or classification of different genotypes. Five populations of human B. hominis isolates obtained from Japa...
Shantanu Roy, Mamun Kabir, Dinesh Mondal, Ibne Karim M. Ali et al.
We developed a real-time-PCR assay utilizing a molecular-beacon probe for the detection of Entamoeba histolytica and compared its sensitivity to stool antigen detection and traditional PCR. A total of 205 stool and liver abscess pus specimens from patients and controls were used for this purpose, 10...
Chikako Shimokawa, Mamun Kabir, Mami Taniuchi, Dinesh Mondal et al.
BACKGROUND: Entamoeba moshkovskii is prevalent in developing countries and morphologically indistinguishable from pathogenic Entamoeba histolytica and nonpathogenic Entamoeba dispar. It is not known if E. moshkovskii is pathogenic. METHODS: Mice were intracecally challenged with the trophozoites of ...
Ibne Karim M. Ali, Utpal K. Mondal, Shantanu Roy, Rashidul Haque et al.
The factors determining whether a person infected with Entamoeba histolytica develops disease remain obscure. To investigate whether the parasite genome contributes to the outcome, we have investigated the distribution of parasite genotypes among E. histolytica-infected individuals in Bangladesh. Sa...
Ibne Karim M. Ali, Shahram Solaymani-Mohammadi, Jasmine Akhter, Shantanu Roy et al.
Entamoeba histolytica infection may have various clinical manifestations. Nine out of ten E. histolytica infections remain asymptomatic, while the remainder become invasive and cause disease. The most common form of invasive infection is amebic diarrhea and colitis, whereas the most common extra-int...
Fahad Aftab, Imran Ahmed, Salahuddin Ahmed, Said M. Ali et al.
BACKGROUND: Maternal morbidity occurs several times more frequently than mortality, yet data on morbidity burden and its effect on maternal, foetal, and newborn outcomes are limited in low- and middle-income countries. We aimed to generate prospective, reliable population-based data on the burden of...
Carol A. Gilchrist, Ibne Karim M. Ali, Mamun Kabir, Faisal Alam et al.
BACKGROUND: The outcome of an Entamoeba histolytica infection is variable and can result in either asymptomatic carriage, immediate or latent disease (diarrhea/dysentery/amebic liver abscess). An E. histolytica multilocus genotyping system based on tRNA gene-linked arrays has shown that genetic diff...
Ibne Karim M. Ali, Rashidul Haque, Abdullah Siddique, Mamun Kabir et al.
BACKGROUND: The category B agent of bioterrorism, Entamoeba histolytica has a two-stage life cycle: an infective cyst stage, and an invasive trophozoite stage. Due to our inability to effectively induce encystation in vitro, our knowledge about the cyst form remains limited. This also hampers our ab...
Ibne Karim M. Ali, Rashidul Haque, Farzana Alam, Mamun Kabir et al.
The Entamoeba histolytica infections result from asymptomatic colonization to variable disease outcomes. However, markers that may predict infection outcomes are not known. Here, we investigated sequence types of a non-coding tRNA-linked locus R-R to identify surrogate markers that may show associat...
Hossain M. S. Sazzad, Stephen P. Luby, James J. Sejvar, Mahmudur Rahman et al.
We present the first recognized case of primary amebic meningoencephalitis (PAM) caused by Naegleria fowleri in a 15-year-old male from Bangladesh. He performed daily nasal rinsing with untreated ground water and bathed in untreated ground water or river water, which likely exposed him to N. fowleri...
Lauren J. Spadafora, Moira R. Kearney, Abdullah Siddique, Ibne Karim M. Ali et al.
Entamoeba histolytica causes intestinal disease in endemic settings throughout the world. Diagnosis of E. histolytica infection would be improved by the identification of biomarkers that are expressed by cysts of E. histolytica, but not by cysts of closely related commensal species of Entamoeba. Her...
Ibne Karim M. Ali, Shantanu Roy
There are over 40 species within the genus Entamoeba , eight of which infect humans. Of these, four species ( Entamoeba histolytica , E. dispar , E. moshkovskii , and E. bangladeshi ) are morphologically indistinguishable from each other, and yet differentiation is important for appropriate treatmen...
Dewan Imtiaz Rahman, Wasik Rahman Aquib, Farzana Fariha, Sopna Choudhury et al.