James B. Kaper, J. Glenn Morris, M. M. Levine
Despite more than a century of study, cholera still presents challenges and surprises to us. Throughout most of the 20th century, cholera was caused by Vibrio cholerae of the O1 serogroup and the disease was largely confined to Asia and Africa. However, the last decade of the 20th century has witnes...
James B. Kaper, S L Moseley, Stanley Falkow
Environmental and nontoxigenic strains of Vibrio cholerae 0-1 were examined for genes homologous to genes encoding Escherichia coli heat-labile enterotoxin (LT). Restriction fragments encoding LT A and B subunits were isolated from the recombinant plasmid EWD299 and labeled in vitro with 32P. These ...
C. O. Tacket, G Losonsky, James P. Nataro, Laurie E. Comstock et al.
Since October 1992, epidemics of cholera associated with Vibrio cholerae O group 139 have occurred in India, Bangladesh, and much of the rest of Asia. A volunteer model was used to determine the safety, immunogenicity, and efficacy of an attenuated delta ctxA delta zot delta ace delta cep V. cholera...
Tracy H. Hazen, Michael S. Donnenberg, Sandra Panchalingam, Martín Antonio et al.
Enteropathogenic Escherichia coli (EPEC) are diarrhoeagenic E. coli, and are a significant cause of gastrointestinal illness among young children in developing countries. Typical EPEC are identified by the presence of the bundle-forming pilus encoded by a virulence plasmid, which has been linked to ...
M. John Albert, Shah M. Faruque, Abu Syed Golam Faruque, K. A. Bettelheim et al.
The role of cytolethal distending toxin (CDT)-producing Escherichia coli, a newly described category of E. coli, in the causation of diarrhea was studied by screening E. coli isolates from 546 children < 5 years of age with diarrhea and 215 matched controls without diarrhea by using a specific DNA p...
L A McNicol, Kanwal Aziz, I Huq, James B. Kaper et al.
Antibiotic-resistant strains of Aeromonas hydrophila have been isolated from the natural environment in the Chesapeake Bay and areas surrounding Dacca and the Matlab region of Bangladesh. The Bangladesh strains carried resistance to chloramphenicol, streptomycin, and tetracycline, and 57% of them ha...
Shah M. Faruque, Laurie E. Comstock, James B. Kaper, Albert Mj
Seventy-two clinical isolates of Vibrio cholerae O1 from Bangladesh, and 12 and 9 isolates respectively from Tanzania and Nigeria were screened for sequences homologous to zonula occludens toxin (zot) and cholera toxin (ctx) genes. As observed previously, all isolates in the present study also posse...
L A McNicol, James B. Kaper, Hank Lockman, Elaine F. Remmers et al.
Two group F vibrio organisms have been identified among a collection of vibrio strains isolated from the aquatic environment in Bangladesh. Neither group F strain produced a cholera-like enterotoxin. One of the isolates, BV12, contained an R plasmid conferring resistance to streptomycin and chloramp...
L A McNicol, S.P. De, James B. Kaper, P. A. West et al.
A total of 165 strains of vibrios isolated from clinical and environmental sources in the United States, India, and Bangladesh, 11 reference cultures, and 4 duplicated cultures were compared in a numerical taxonomic study using 83 unit characters. Similarity between strains was computed by using the...
Article Figures and data Abstract eLife digest Main Text Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciate...